从蝙蝠便样本中分离出的Enterococcus faecium菌株的有益特性
Tatiana Alexandrovna Lipilkina1,2,3, Marcos Vinício Alves1, Kayque Ordonho Carneiro1
1ProBacLab, Laboratório de Microbiologia de Alimentos, Departamento de Alimentos e Nutrição Experimental, Food Research Center, Faculdade de Ciências Farmacêuticas, Universidade De São Paulo, São Paulo, Brazil.
Molecular nutrition & food research
|August 30, 2025
概括
蝙蝠便含有具有益生菌潜力和抗菌性能的Enterococcus faecium菌株 这些新型菌株对食品安全和肠道健康有前途,但需要进一步的安全评估.
科学领域:
- 微生物学
- 益生菌
- 食品安全问题
背景情况:
- 菌 (E. faecium) 是一种常见的细菌,具有已知的益生菌和致病潜力.
- 蝙蝠,特别是夜,越来越多地被认为是新型微生物的潜在储存库.
研究的目的:
- 为了隔离和表征E. faecium从Nyctalus noctula蝙蝠便.
- 评估已分离的E. faecium菌株的益生菌潜力和抗菌特性.
- 评估这些菌株在食品安全和肠道健康方面的安全性和潜在应用.
主要方法:
- 采集Nyctalus noctula蝙蝠的便样本
- 对Listeria monocytogenes的抗菌活性进行查.
- 使用复制PCR指纹和16S rRNA测序进行细菌鉴定.
- 确认细菌素的产生和稳定性测试 (pH,温度,化学条件).
- 评估益生菌特征 (疏水性,酶活性) 和毒性因子 (溶血性,粘素降解活性).
- 对细菌素和GABA合成基因进行分子分析.
主要成果:
- 分离了两种产生细菌素的E. faecium菌株 (ST01TL和ST76TL).
- 抗菌物质是蛋白质的,在各种条件下稳定,对Listeria monocytogenes有效.
- 菌株表现出可取的益生菌特征,如疏水性和有益的酶活性,没有血液溶解或粘素降解.
- 检测到了细菌素和GABA合成的基因.
- 鉴定出有限的毒性特征和抗生素耐药性,需要进一步调查.
结论:
- 尼克塔卢斯·诺克图拉蝙蝠的E. faecium菌株具有显著的益生菌潜力和稳定的抗菌活性.
- 这些菌株可能对食品安全和改善肠道健康有价值.
- 蝙蝠是益生菌发现的新来源,但在应用之前,包括全基因组测序在内的全面安全评估至关重要.
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