在Botrytis cinerea中通过多策略组合优化进行高水平的酸生产
Ling-Ru Wang1, Shu-Ting Zhu1, Ji-Zi-Hao Tang1
1School of Food Science and Pharmaceutical Engineering, Nanjing Normal University, Nanjing, Jiangsu, China.
Biotechnology and bioengineering
|August 30, 2025
概括
这项研究通过优化其应激防御和辅助因子供应,增强了Botrytis cinerea的酸 (ABA) 生产. 这使得ABA产量增加了76%,提高了工业生物加工的效率.
科学领域:
- 生物技术
- 菌群学
- 植物生理学
背景情况:
- 酸 (ABA) 是一种具有广泛应用的重要植物激素.
- 使用Botrytis cinerea的工业ABA合成面临着重大生产挑战.
研究的目的:
- 为了增强Botrytis cinerea的ABA生物合成能力.
- 克服工业ABA发酵的局限性
主要方法:
- 多策略优化包括应力防御调节和辅助因子供应增强.
- 补充抗氧化化合物 (例如,视网醇) 和体内代谢工程.
- 优化发酵条件
主要成果:
- 雷丁醇补充剂增加了ABA产量至1.22g/L.
- 转录和生物化学分析证实了压力防御的改善.
- 优化发酵条件导致ABA产量增加了76% (1.72g/L).
结论:
- 建立了一个强大的方法,以显著增强B. cinerea中的ABA生物合成.
- 这些发现为改善状真菌的工业生物处理提供了宝贵的见解.
- 这种工作为更高效的种植和生产铺平了道路.
相关概念视频
Adaptations that Reduce Water Loss
26.3K
Though evaporation from plant leaves drives transpiration, it also results in loss of water. Because water is critical for photosynthetic reactions and other cellular processes, evolutionary pressures on plants in different environments have driven the acquisition of adaptations that reduce water loss.
26.3K
C4 Pathway and CAM
46.2K
Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
C4 Pathway
The C4 pathway is used by plants such as...
46.2K
The Calvin Benson Cycle
4.7K
Ribulose 1,5- bisphosphate carboxylase/oxygenase (RuBisCo) is a critical enzyme that catalyzes carbon dioxide assimilation during photosynthesis. However, it is an inefficient enzyme, having an extremely slow catalytic rate. A typical enzyme can process about a thousand molecules per second; however, RuBisCo fixes only around three-carbon dioxides per second. Photosynthetic cells compensate for this slow rate by synthesizing very high amounts of RuBisCo, making it the most abundant single...
4.7K


