作为高性能脂质酶固定的先进支持的氨基化木质素介质碳
Xing Ni1, Luyi Chen1, Xue Zhang1
1School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China.
Colloids and surfaces. B, Biointerfaces
|August 30, 2025
概括
性木质素被修改并用于制造酶固定化的中孔碳载体. 氨基化木质素衍生载体 (IM-AAL-MC) 显示出优异的酶活性,负载能力和稳定性,特别是在有机溶剂中.
科学领域:
- 生物技术
- 材料科学
- 生物聚合物工程
背景情况:
- 性素 (AL) 是一种丰富的可再生生物聚合物,具有作为先进材料的前体的潜力.
- 开发高效的酶固定化策略对于生物催化和工业应用至关重要.
- 半孔碳 (MC) 材料具有高表面积和可调节的酶支持特性.
研究的目的:
- 从改造的性木质素合成新型的中孔碳载体,用于酶固定.
- 对这些载体的固定Rhizomucor miehei脂酶 (RML) 的作用进行研究.
- 评估固定酶的催化效率,负载能力和稳定性,特别是IM-AAL-MC.
主要方法:
- 性红素被化学修饰以产生氨基化 (AAL),硫甲基化 (SML) 和化 (PAL) 红素.
- 使用纳米MgO作为模板,并碳化素/纳米MgO混合物产生半孔碳载体 (AL-MC,AAL-MC,SML-MC,PAL-MC).
- 通过物理吸附将Rhizomucor miehei脂酶 (RML) 固定在载体上,然后使用FTIR,XRD,BET和TEM进行表征.
主要成果:
- 合成的半孔碳载体证明适用于RML固定.
- IM-AAL-MC 具有较高的酶活性 (0. 037 U/ mg),负载能力 (0. 145 mg/ mg) 和固定效率 (84. 5%).
- 在六个重复使用周期后,IM-AAL-MC表现出了显著的稳定性,在n- hexane中保持了99. 49%的活性,而在自由脂酶中保持了43. 53%的活性.
结论:
- 改性性是一种有效的碳酶载体的可行前体.
- 氨基化木质素衍生的半孔碳载体 (IM-AAL-MC) 提供增强的酶活性,负载和特殊的稳定性,特别是在非水性环境中.
- 这项研究提出了一种可持续的方法,用于开发各种应用的高性能固定酶.
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