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一种自动化免疫亲和液体染色体-双重质谱试验,用于测量人体血中的阿尔多

Liang-Xing Li¹, Jian-You Xue², Wan-Ying Lin¹

¹Department of Laboratory Medicine, Guangdong Provincial Key Laboratory of Precision Medical Diagnostics, Guangdong Engineering and Technology Research Center for Rapid Diagnostic Biosensors, Guangdong Provincial Key Laboratory of Single-cell and Extracellular Vesicles, Nanfang Hospital, Southern Medical University, Guangzhou, China.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences

|August 30, 2025

概括

通过精确测量阿尔多斯特,新的自动化测试显著加快了原发性阿尔多斯特症的诊断. 这种免疫 afinity-mass spectrometry (iMS) 方法为传统的实验室技术提供了更快,更高的输出率的替代方案.

科学领域:

生物化学
分析化学
临床诊断

背景情况:

准确的阿尔多测量对于诊断原发性阿尔多 (PA) 是至关重要的.
液体染色体-双重质谱 (LC-MS/MS) 是黄金标准,但需要大量的样本准备.
需要更快,自动化方法来量化阿尔多.

研究的目的:

开发和验证一种完全自动化的免疫 afinity-mass spectrometry (iMS) 测定阿尔多.
与现有方法相比,评估自动化iMS测试的性能和效率.

主要方法:

使用抗阿尔多抗体和稳定同位素标记的内部标准的自动化iMS测试的开发.
在完全自动化的装置上进行测试,手动干预最小.
评估关键性能指标,包括量化极限 (LOQ),回收,线性和精度.

主要成果:

通过iMS测定,获得了50 pg/ ml的LOQ和高回收率 (105. 1113. 9%).
显示出优异的线性 (R2 = 0. 9993) 和测试间精度 (CV 2. 33 3. 91%).
在iMS和免疫检测结果之间观察到高相关性 (R=0. 947).

结论:

自动化iMS平台为阿尔多测量提供临床级别的灵敏度,精度和可扩展性.
这种高通量方法大大缩短了样本准备时间,使其适用于常规的PA测试.
该平台可适应其他临床分析物的量化.

关键词:

阿尔多斯特自动化免疫亲和一个LC-MS/MS 主要的阿尔多斯特主义

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