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Updated: Sep 9, 2025

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在空气-液体接口培养下使用人类iPSC模拟肠上皮功能和极性

Eri Tanaka1, Takahiro Kishikawa1, Yu Miyakawa1

  • 1Department of Gastroenterology, Graduate School of Medicine, The University of Tokyo, Tokyo, 113-8655, Japan.

Biochemical and biophysical research communications
|August 30, 2025
PubMed
概括

使用人类诱导的多能干细胞 (iPSC) 的新型空气-液体接口 (ALI) 培养系统产生极化肠表皮. 这种模型可以研究药物反应和细胞外囊 (EV) 的分泌,从而促进肠道生物学研究.

关键词:
空气液体接口培养细胞外囊泡诱导多能干细胞肠道细胞

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The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
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