相关实验视频

Updated: May 10, 2026

Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates

Stencil Micropatterning of Human Pluripotent Stem Cells for Probing Spatial Organization of Differentiation Fates

Published on: June 17, 2016

有孔的微支架可使血造细胞自发分化,并从hPSC中持续产生巨细胞进行治疗

Peiliang Wang¹, Hui Qiu¹, Xia Chen²

¹Center for Regeneration, Aging and Chronic Diseases, School of Basic Medical Sciences, Tsinghua University, Beijing, 100084, China; SXMU-Tsinghua Collaborative Center for Frontier Medicine, Taiyuan, Shanxi Province, 030001, China.

|August 30, 2025

概括

一种新型的多孔微支架 (PMS) 便于从人类多能干细胞中进行可扩展的功能性巨细胞的产生. 这一突破有助于开发用于感染和其他疾病的现成细胞疗法.

科学领域:

干细胞生物学
细胞和分子医学
复原医学

背景情况:

巨细胞对健康和疾病至关重要,
从干细胞生成巨细胞的现有方法通常需要大量的细胞因子使用,并且缺乏可扩展性.

研究的目的:

开发一种可扩展且具有成本效益的方法,从人类多能干细胞 (hPSC) 产生功能性巨细胞.
研究新型多孔微支架 (PMS) 增强巨细胞分化和扩张的机制.

主要方法:

人类多能干细胞衍生的中皮在多孔微支架 (PMS) 上培养.
分析了PMS中的中皮细胞自组织成大动脉 - 淋巴细胞 - 淋巴细胞 (AGM) 类似的.
用内皮细胞修饰的PMS来研究膜信号和基因激活.
进行多组基因分析以确定参与巨扩张的关键基因网络.
在药物耐药性Streptococcus pneumoniae感染的小鼠模型中评估了PMS衍生的巨细胞的治疗疗效.

主要成果:

PMS促进了来自hPSC的强大的造血分化.
中皮细胞在PMS内自组织成血管结构,模仿AGM,增强内皮转化为造血细胞.
由内皮细胞修饰的PMS增加了膜信号,促进了巨细胞的增殖和自我更新.
在3D微环境中增强了巨细胞扩张的基因网络.
在体内,PMS衍生的巨体对抗耐药细菌感染具有显著的治疗潜力.

结论:

多孔微支架 (PMS) 提供了一个具有成本效益和可扩展的平台,用于从hPSCs生成功能性巨细胞.
这种方法克服了当前巨细胞生产的局限性,使得可以开发现成的基于细胞的疗法.
这些发现突显了工程微环境在治疗应用中直接分化干细胞的潜力.

关键词:

三维培养人类多能干细胞巨细胞微型架构

更多相关视频

Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition

Hemogenic Endothelium Differentiation from Human Pluripotent Stem Cells in A Feeder- and Xeno-free Defined Condition

Published on: June 16, 2019

Production and Characterization of Human Macrophages from Pluripotent Stem Cells

Production and Characterization of Human Macrophages from Pluripotent Stem Cells

Published on: April 16, 2020

相关概念视频

Hematopoiesis

Hematopoiesis

The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...

Multipotency of Hematopoietic Stem Cells

Multipotency of Hematopoietic Stem Cells

The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...

Regulation of Hematopoietic Stem Cells

Regulation of Hematopoietic Stem Cells

All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...