PFBS 破坏了人体热原体细胞中的脂质代谢和线粒体功能
Julia Happel1, Namya Mellouk2, Christine Crute1
1Nicholas School of the Environment, Duke University, Durham, NC, USA.
Toxicology
|August 30, 2025
概括
通过扰乱脂质代谢和能量产生,对胎盘细胞造成伤害. 这种线粒体功能障碍可能会将PFBS暴露与不良妊娠结果联系起来.
科学领域:
- 环境健康
- 生殖毒理学
- 线粒体生物学
背景情况:
- 和多基物质 (PFAS) 是与不良生殖结果相关的持续性环境污染物.
- 胎盘对于怀孕至关重要,
- 胎盘中的线粒体功能对胎儿和胎盘生长至关重要,并且干扰可以调解环境暴露的影响.
研究的目的:
- 研究甲酸 (PFBS) 对人体同胞细胞 (STB) 代谢和线粒体功能的影响.
- 探索PFBS可能影响胎盘健康和妊娠结果的分子机制.
主要方法:
- 人类多细胞干细胞分化为STB,并暴露于环境相关的PFBS剂量 (100μM).
- 进行了转录组 (RNA-seq) 和代谢组分析以评估基因表达和代谢变化.
- 通过对线粒体DNA复制数,ATP产生,氧化应激和形态的评估来评估线粒体功能.
主要成果:
- PFBS暴露显著破坏了脂质代谢,降低了参与脂肪酸进口的PPARG,CPT1A和SLC25A20等关键基因.
- 线粒体功能障碍是由线粒体DNA复制数的增加和ATP产量的减少表明的.
- 在STB中,PFBS没有引起显著的氧化应激或改变线粒体形态.
结论:
- PFBS主要通过扰乱脂质代谢和线粒体能量产生,诱导人类的性传播细胞的代谢毒性.
- 这些发现提供了将PFBS暴露与胎盘功能障碍和不良妊娠结果联系在一起的潜在机制.
- 针对脂质代谢和线粒体功能可能对减轻PFBS对怀孕的不良影响至关重要.
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