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Bioreactor Design and Operational System01:29

Bioreactor Design and Operational System

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Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
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Bioreactor Controls-II01:18

Bioreactor Controls-II

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In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the...
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Designing Growth Media for Bioreactors01:30

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Growth media provide essential nutrients that support cell growth and metabolism, thereby enhancing the yield of valuable products such as enzymes, antibiotics, and biomass. Designing an effective growth medium involves balancing all components to prevent nutrient limitations or toxic excesses, both of which can impair growth and reduce product yields.Composition of a Typical Growth MediumA typical growth medium contains carbon and nitrogen sources, salts, vitamins, trace elements, and...
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Scale-Up Processes01:14

Scale-Up Processes

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The scale-up of microbial fermentation processes is essential in industrial biotechnology, allowing the transition from laboratory-scale experiments to commercial-scale production while aiming to maintain product yield and quality. This process requires meticulous adjustment of equipment design, process parameters, and contamination control strategies to accommodate increasing culture volumes.At the laboratory scale, cultures are typically maintained in 1 to 10-liter glass or autoclavable...
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Upstream Processing01:27

Upstream Processing

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Upstream processing represents a critical phase in biomanufacturing, wherein biological systems such as microorganisms, mammalian cells, or insect cells are cultivated to produce therapeutic proteins, vaccines, enzymes, or other biologically derived products. This phase encompasses all steps from the selection and genetic manipulation of the production organism to the cultivation of cells in bioreactors under tightly controlled environmental conditions.Host Selection and Genetic OptimizationThe...
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Persistent low PHB accumulation during pilot-scale gas fermentation in Cupriavidus necator.

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用Cupriavidus necator H16进行SCP生产的试验规模压力深喷气生物反应器的性能

Kay Domenico Novak1, Petra Heidinger1, Daniel Schwendenwein1

  • 1acib GmbH, Krenngasse 37, Graz A-8010, Austria.

Journal of biotechnology
|August 30, 2025
PubMed
概括

使用Cupriavidus necator H16的化学自营性注射剂显著提高了单细胞蛋白 (SCP) 生产效率. 这种方法绕过了滞后阶段,为可持续的料替代品提供了更快,更可扩展和更高质量的蛋白质产量.

关键词:
化学自营性子子气体发酵高细胞密度试点工厂单细胞蛋白

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科学领域:

  • 生物技术和工业微生物学
  • 可持续的蛋白质生产
  • 化学工程

背景情况:

  • 全球对蛋白质的需求正在增加,
  • 单细胞蛋白 (SCP) 为蛋白质供应提供了一个有前途的解决方案.
  • 优化发酵过程是工业规模SCP生产的关键.

研究的目的:

  • 评估Cupriavidus necator H16在SCP生产中的工业潜力.
  • 调查注射型对发酵效率和可扩展性的影响.
  • 评估SCP在料应用中的质量和适用性.

主要方法:

  • 使用一个定制的300升压力深喷气生物反应器,
  • 使用化学自与异性注射剂的发酵性能比较.
  • 监测的细胞湿量 (CWW),细胞干量 (CDW) 和气体转移/消耗率.

主要成果:

  • 化学自营性注射剂导致了即时的生产力,消除了异构性注射剂所见的滞后阶段.
  • 在35小时内达到300g/L的CWW,使用化学自营性注射剂,比异构性更快44%.
  • 通过达到360 g/L CWW (90 g/L CDW) 和高气体转移率 (H2:0.82 kg/h,O2:4.1 kg/h,CO2:1.95 kg/h) 证明了可扩展性.

结论:

  • 在工业上,Cupriavidus necator H16可用于高质量的SCP生产.
  • 对于优化发酵效率和产量而言,无菌选择至关重要.
  • 产生的SCP含有80%的蛋白质和平衡的氨基酸,是水产养殖和家禽料的可持续替代品.