分子流行病学和基因组洞察 肠道菌综合体 携带blaKPC和mcr:对抗性基因传播的影响
Boqian Wang1, Jingjing Fu2, Yaqing He3
1Laboratory of Advanced Biotechnology, Beijing Institute of Biotechnology, Beijing 100071, China.
International journal of antimicrobial agents
|August 30, 2025
概括
菌菌复杂菌株是blaKPC和mcr基因的储存库,通常位于IncHI2(2A) 质粒上. 这凸显了在临床环境中监测这些共同存在的耐药基因的必要性.
科学领域:
- 分子流行病学
- 基因组表征
- 抗菌药物耐药性
背景情况:
- 肠道菌复合体 (ECC) 菌株是重要的机会性病原体.
- 携带碳烯 (blaKPC) 和胆素 (mcr) 耐药基因的出现对公众健康构成严重威胁.
- 了解共抗的分子基础对于有效的治疗策略至关重要.
研究的目的:
- 系统地研究含有blaKPC和mcr基因的ECC菌株的分子流行病学和基因特征.
- 分析这些耐药性决定因素的共存和遗传背景.
- 为监测和控制多药性ECC的传播提供见解.
主要方法:
- 从NCBI GenBank数据库 (2003-2024) 获取全基因组测序数据的281个ECC分离物的分析.
- 对ECC菌株进行基因分析以了解进化关系.
- 使用ABRicate和CARD数据库对抗性基因的识别和分布分析.
- 使用PlasmidFinder对blaKPC和mcr基因周围遗传环境进行复制和分析.
主要成果:
- 主要的基因组合是blaKPC-3和mcr-9.1,在E. hormaechei ssp中经常发现. 花科植物.
- 主要检测到来自美国的分离物中携带blaKPC和mcr-9的IncHI2(2A) 质粒.
- 遗传学分析表明,携带mcr-9的IncHI2(2A) 质体可能已经获得了blaKPC,从而促进了同时传播.
结论:
- ECC菌株是blaKPC和mcr-9共同传播的重要储存体.
- IncHI2 ((2A) 等离子体作为可水平转移碳烯和素抗性的潜在载体.
- 有效的监测是监测blaKPC和mcr-9携带的ECC分离物和等离体的必要条件.
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