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相关概念视频

Photoreceptors and Visual Pathways01:22

Photoreceptors and Visual Pathways

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At the molecular level, visual signals trigger transformations in photopigment molecules, resulting in changes in the photoreceptor cell's membrane potential. The photon's energy level is denoted by its wavelength, with each specific wavelength of visible light associated with a distinct color. The spectral range of visible light, classified as electromagnetic radiation, spans from 380 to 720 nm. Electromagnetic radiation wavelengths exceeding 720 nm fall under the infrared category,...
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The Retina01:32

The Retina

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The retina is a layer of nervous tissue at the back of the eye that transduces light into neural signals. This process, called phototransduction, is carried out by rod and cone photoreceptor cells in the back of the retina.
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这页已由机器翻译。其他页面可能仍然显示为英文。View in English
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  6. 在盲人视网膜中使用超敏感的人类trpv1通道进行红外探测

在盲人视网膜中使用超敏感的人类TRPV1通道进行红外探测

Morgan Chevalier1, Firas Fadel1, Tímea Májer2

  • 1Institute of Molecular and Clinical Ophthalmology Basel, Basel, Switzerland.

Trends in biotechnology
|August 30, 2025

相关实验视频

Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina
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Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina

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Transretinal ERG Recordings from Mouse Retina: Rod and Cone Photoresponses
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Transretinal ERG Recordings from Mouse Retina: Rod and Cone Photoresponses

Published on: March 14, 2012

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Electrophysiological Characterization of GFP-Expressing Cell Populations in the Intact Retina
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Electrophysiological Characterization of GFP-Expressing Cell Populations in the Intact Retina

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在PubMed 上查看摘要

概括
此摘要是机器生成的。

科学家们设计了人类的TRPV1通道以响应近红外光线, 这为盲人恢复视力提供了潜在的途径. 这种单组件,人源的方法避免了免疫反应,并简化了视网膜退化的临床转换.

科学领域:

  • 生物医学工程
  • 视觉遗传学
  • 神经科学

背景情况:

  • 在视网膜退化中恢复视力是一项重大挑战.
  • 目前的光遗传策略往往涉及复杂的非人类成分,引发了免疫性和临床转化问题.

研究的目的:

  • 设计一种人为的视力修复工具.
  • 在生理温度下使近红外光激活视网膜细胞.

主要方法:

  • 使用合理蛋白质设计来修改人类TRPV1通道 (hTRPV1).
  • 设计的hTRPV1 (Δ786-840) 显示出温度灵敏度的转移,使其能够在生理温度附近激活.
  • 该通道在人类视网膜质细胞中表达,并对近红外光的反应进行了测试.

主要成果:

  • 在接近生理温度的近红外光下激活hTRPV1通道.
  • 人类视网膜扩展剂的表达产生了强烈的NIR光反应.
  • 响应与增加的光强度进行分级,与发射频率相关联.

结论:

  • 这种单个成分的人类hTRPV1提供了一个有前途的,免疫安全的视力恢复方法.
  • 该方法通过腺相关病毒 (AAV) 载体简化基因传递,解决了关键的临床转化障碍.
关键词:
TRPV频道视网膜细胞中的电生理学工程离子通道基因疗法

相关实验视频

Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina
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Transpupillary Two-Photon In Vivo Imaging of the Mouse Retina

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Transretinal ERG Recordings from Mouse Retina: Rod and Cone Photoresponses
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Transretinal ERG Recordings from Mouse Retina: Rod and Cone Photoresponses

Published on: March 14, 2012

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Electrophysiological Characterization of GFP-Expressing Cell Populations in the Intact Retina
07:30

Electrophysiological Characterization of GFP-Expressing Cell Populations in the Intact Retina

Published on: November 14, 2011

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人类视网膜
光热刺激
蛋白质工程
视力恢复