在小鼠中改善工程精氨酸分泌Saccharomyces boulardii的结肠炎和结肠癌
Mahdi Mohaqiq1, Roger Palou2, Ruizhen Li1
1Ottawa Hospital Research Institute, Ottawa, ON, K1H 8L6, Canada.
Scientific reports
|August 30, 2025
概括
在小鼠中,分泌精氨酸的工程酵母Saccharomyces boulardii有效降低了炎症性肠道疾病和相关的结肠癌. 这种合成生物方法为胃肠道疾病提供了有前途的治疗策略.
科学领域:
- 微生物学
- 胃肠病学
- 生物技术
背景情况:
- 益生菌酵母Saccharomyces boulardii具有炎症性肠病 (IBD) 的潜力,但缺乏临床接受.
- 目前用于IBD和相关结肠癌的治疗方法存在局限性.
研究的目的:
- 增强S. boulardii的治疗效果,使其过度产生和分泌精氨酸.
- 评估分泌精氨酸的S. boulardii作为治疗肠道炎症和癌症的潜力.
主要方法:
- 在S. boulardii中利用CRISPR基因编辑进行高水平的聚胺合成和运输.
- 通过口服分泌精氨酸的S. boulardii在小鼠模型中使用,该小鼠模型中使用了硫酸 (DSS) 诱导的结肠炎和氧甲诱导的致癌症.
- 通过工程酵母评估胃肠道的殖民性,并测量自由精氨酸水平.
主要成果:
- 通过口服输送成功填充了肠道有活力的精素分泌S. boulardii,并增加了精素水平.
- 与野生型S. boulardii相比,工程菌株显著降低了结肠炎症状.
- 在小鼠中,分泌精子素的S. boulardii在降低大肠炎相关的致癌性方面表现出卓越的有效性.
结论:
- 人工酵母在现场分泌精子是一种新的治疗策略.
- 这种合成生物方法可以为IBD和结肠癌提供有效且低成本的治疗方法.
- 对胃肠健康的工程益生菌进行进一步的研究是有必要的.
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