增强的表面区域慢性细胞扩张和通过培养在慢性细胞衍生的脱细胞矩阵上的再分化
Thomas J Manzoni1, Anh Ho1, Lilly Smull1
1Department of Biological Sciences, University of Delaware, Newark, DE, USA.
Cartilage
|August 31, 2025
概括
在冠状细胞衍生的脱细胞外基质 (CD-ECM) 上培养表面区域冠状细胞 (SZC) 可以改善细胞的附着和增殖. 这种方法增强了具有更高蛋白质甘氨酸-4 (PRG4) 表达的工程软骨的形成.
科学领域:
- 生物材料科学
- 组织工程
- 细胞生物学
背景情况:
- 软骨再生疗法在复制本地组织结构和功能方面面临挑战.
- 经过临床认可的细胞来源,通常在工程组织中表现出减少的蛋白质甘氨酸-4 (PRG4).
- 表面区冠状细胞 (SZC) 的附着性较差,在标准培养面上不分化,阻碍了它们的使用.
研究的目的:
- 对SZC附着,增殖和脱差的冠状细胞衍生脱细胞化外基质 (CD-ECM) 的影响进行调查.
- 确定CD-ECM培养条件是否可以提高SZC形成PRG4表达生物工程软骨的能力.
主要方法:
- 在聚乙烯或CD-ECM上培养初级牛肉SZC.
- 在传递过程中监测了细胞附着,增殖率和基因表达.
- 评估了扩张SZC形成PRG4表达软骨的能力.
主要成果:
- 在CD-ECM上培养的SZC显示了显著增加的附着和更快的合流性.
- CD-ECM上的细胞较小,表现出较少的动因应变纤维,并显示出减少的脱差标记.
- 在CD-ECM上扩展的SZC形成的工程组织富含原II和Aggrecan,具有增强的PRG4表达.
结论:
- CD-ECM为SZC扩张提供支持的微环境,改善细胞行为.
- 使用CD-ECM进行SZC传递是产生足够细胞用于生物工程软骨的有希望的策略.
- 这种方法有助于创建具有改善区域特性的工程软骨,包括更高的PRG4表达.
相关概念视频
01:22Enlargement of the Plasma Membrane
1.8K
Cell division and enlargement are processes that require precise control. The control ensures that cell division cannot proceed unless the cell has grown to a specific size. A spherical, dividing cell requires an approximately 1.6X increase in its surface area to double its volume. The secretory pathway also has a significant role in cell membrane enlargement. Secretory vesicles that bud off from the Golgi apparatus and later fuse with the plasma membrane during exocytosis are a major source of...
1.8K
01:26Stem Cell Niche
5.0K
The stem cell niche is the dynamic microenvironment where stem cells reside. Inside these niches, the cells may remain undifferentiated, undergo high self-renewal, or become lineage-specific progenitors. Stem cells coexist with other niche cells, such as stromal cells. They also interact closely with the ECM. Cell-cell and cell-matrix communication occur via adhesion molecules or soluble factors that signal the stem cells and determine their fate. Stromal cells also provide survival signals to...
5.0K
01:23Renewal of Intestinal Stem Cells
2.7K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
2.7K
01:12Renewal of Skin Epidermal Stem Cells
2.4K
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...
2.4K
01:23Tissue Renewal without Stem Cells
1.6K
After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...
However, failure of such a system...
1.6K
01:26Cellular Adaptation III: Hyperplasia
75
Hyperplasia is an increase in the number of cells in a tissue or organ due to enhanced cell division. It is an adaptive, controlled response to stimuli such as injury, hormones, or stress, involving mitosis to produce genetically identical cells and support tissue repair and regeneration.Tissue CapacityCertain tissues, including the epidermis, intestinal epithelium, bone marrow, and fibroblasts, have a high potential for hyperplasia. Others, such as bone, cartilage, and smooth muscle, show...
75