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Updated: Sep 9, 2025

Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

使用CircRNAFlow进行循环RNA识别和表征:一种生物信息学方法

Edward A Salinas¹, Yvonne J K Edwards^2,3,4

¹Department of Biochemistry and Molecular Genetics, Heersink School of Medicine, University of Alabama at Birmingham, Birmingham, AL, USA.

Advances in experimental medicine and biology

|August 31, 2025

概括

CircRNAFlow是一个新的生物信息管道,用于从测序数据中识别和表征循环RNA (circRNA). 这种工具有助于研究人员了解circRNAs在基因调节和疾病中的作用.

科学领域:

基因组学
生物信息学
分子生物学

背景情况:

循环RNAs (circRNAs) 在基因调节和疾病发展中至关重要.
circRNAs 作为微RNA海绵,与RNA结合蛋白相互作用,并且可以被翻译.
这些共价封闭的环状结构的特征仍然有限.

研究的目的:

介绍CircRNAFlow,一个免费的,开源的Nextflow管道,用于in silicocircRNA的识别和表征.
使用集成的第三方软件提供circRNA的综合生物信息分析.
提供CircRNAFlow安装,执行和输出解释的教程.

主要方法:

使用下一代测序 (NGS) 处理结对的FASTQ文件.
该管道整合了诸如FastQC,Flexbar,Bowtie2,STAR,DCC,CircTest,CRAFT,ClusterProfiler和deepTarget等工具.
输出包括表达比较报告和已识别的circRNA的功能特征.

主要成果:

CircRNAFlow生成表格和图形,详细说明已识别的circRNA及其特征.
报告允许对宿主基因和实验组之间的circRNA表达进行比较.
功能性表征通过CRAFT和deepTarget等工具来探索监管作用.

结论:

CircRNAFlow提供了一个强大且易于使用的circRNA分析平台.
这条管道有助于更深入地了解生物过程和疾病中的circRNA功能.
这个资源与GitHub存储库相结合,支持circRNA生物信息学研究人员.

关键词:

在CircRNAFlow中环形RNA 微RNA海绵下一个流结合RNA的蛋白位点在RNA-seq 翻译模板工作流程的发展

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