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Updated: Sep 9, 2025

Identification of Circular RNAs using RNA Sequencing

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

循环RNA的形成

Arvind Srinivasan¹, Marzena Wojciechowska²

¹Department of Rare Diseases, Polish Academy of Sciences, Institute of Bioorganic Chemistry, Poznan, Poland.

Advances in experimental medicine and biology

|August 31, 2025

概括

循环RNAs (circRNAs) 是由背接形成的独特RNA分子. 它们的生物发生包括cis作用元素和RNA结合蛋白,影响细胞功能.

科学领域:

分子生物学
遗传学
核糖核酸生物学

背景情况:

循环RNAs (circRNAs) 是通过背接形成的非线性RNA分子.
由于它们的闭环结构,它们与线性RNA不同.
循环RNAs通过各种机制产生,包括直接回,异位跳转和拉里亚特跳转.

研究的目的:

审查影响circRNA生物发生的变量.
突出不同类型的人类circRNA的起源.
阐明控制circRNA合成的调节机制.

主要方法:

对circRNA生物发生现有文献的审查.
对参与circRNA形成的cis-acting元素和trans-acting因素的分析.
检查反向补充匹配 (RCM) 和RNA结合蛋白 (RBP) 的作用.

主要成果:

循环RNA生物发生是由 cis 作用元素和 trans 作用因子的复杂相互作用调节的.
反向补充匹配 (RCM) 便于对拼接部位进行反向拼接.
通过影响背接效率,RNA结合蛋白 (RBPs) 可以调节circRNA合成.
存在三种主要类型的circRNA:外源性 (EcircRNAs),内源性 (IcircRNAs) 和外源性-内源性circRNAs (EIcircRNAs).

结论:

循环RNA合成是一个严格调节的过程,对细胞活动至关重要.
了解circRNA生物发生是理解它们多样性的关键.
遗传元素和蛋白质因素的相互作用塑造了circRNA生产的格局.

关键词:

背接方式循环RNA 互联网监管要素核糖核酸拼接交互作用的因素

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In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

In Silico Identification and Characterization of circRNAs During Host-Pathogen Interactions

Published on: October 21, 2022

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Use of Alu Element Containing Minigenes to Analyze Circular RNAs

Published on: March 10, 2020

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