相关实验视频

Updated: Sep 9, 2025

Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions

Caffeine Extraction, Enzymatic Activity and Gene Expression of Caffeine Synthase from Plant Cell Suspensions

Published on: October 2, 2018

来自Acorus calamus的咖啡酸O-甲基转移酶的分子克隆和特征

Takao Koeduka¹, Koki Aono¹, Kanon Goto¹

¹Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Yamaguchi, 753-8515, Japan.

Journal of plant physiology

|August 31, 2025

概括

研究人员在甜植物中发现了一种新型O-甲基转移酶. 这种酶AcCOMT在产生特征性香味方面发挥作用,但在进化上与相似的植物酶不同.

科学领域:

植物生物化学
酵素学
分子进化

背景情况:

像α-和β-asarone这样的挥发性烯是药用甜旗 (Acorus calamus) 的关键香味.
对于A. calamus中挥发性烯生物合成的特定酶的知识有限.

研究的目的:

鉴定和描述涉及A. calamus中挥发性烯生物合成的酶.
研究一种新发现的O-甲基转移酶的酶活性和进化关系.

主要方法:

在A. calamus中识别候选基因的RNA测序.
在大肠杆菌中发现的S-adenosyl-L-methionine依赖O-methyltransferase (AcCOMT) 的重组表达.
酶测试以确定基质的特异性.
遗传学分析以评估进化关系.

主要成果:

鉴定了一种编码S-adenosyl-L-methionine依赖O-methyltransferase (AcCOMT) 的基因.
重组AcCOMT特异地甲基化了咖啡酸的甲基基,5- 基酸和5- 基醇.
AcCOMT对甲基醇类型的 stilbenes,flavonoids或可疑的前体无活性.
遗传学分析表明AcCOMT与其他植物COMT有着遥远的进化关系.

结论:

在A. calamus中,AcCOMT参与了甲的甲基化途径.
酶的独特基质特异性和进化差异表明它具有不同的功能作用.
与其他O-甲基转移酶相比,AcCOMT是一种早期分离的酶,具有独特的进化轨迹.

关键词:

青树药用植物甲基转移酶甲挥发性化合物

更多相关视频

Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds

Using Capillary Electrophoresis to Quantify Organic Acids from Plant Tissue: A Test Case Examining Coffea arabica Seeds

Published on: November 12, 2016

Transcript and Metabolite Profiling for the Evaluation of Tobacco Tree and Poplar as Feedstock for the Bio-based Industry

Transcript and Metabolite Profiling for the Evaluation of Tobacco Tree and Poplar as Feedstock for the Bio-based Industry

Published on: May 16, 2014

相关概念视频

C4 Pathway and CAM

C4 Pathway and CAM

Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...