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慢性淋巴细胞白血病中的非编码RNA:系统性审查和元分析来解码诊断潜力

Amir Hossein Aghayan¹, Ali Arab², Shadi Haddadi²

¹Student Research Committee, Department of Medical Laboratory Sciences, School of Paramedical Sciences, Zanjan University of Medical Sciences, Zanjan, Iran.

Molecular and cellular probes

|August 31, 2025

概括

微RNAs (miRNAs) 和循环RNAs (CircRNAs) 显示为诊断慢性淋巴细胞白血病 (CLL) 的非侵入性生物标志物. 虽然MiRNA的诊断准确度更高,但需要进一步的研究来证实这些发现,以确定CLL的诊断.

科学领域:

血液学
分子生物学
癌症学

背景情况:

慢性淋巴细胞白血病 (CLL) 是西方国家常见的白血病.
非编码RNAs (ncRNAs),包括miRNAs,lncRNAs和CircRNAs,在CLL的发展和调节中发挥作用.
作为CLL诊断的非侵入性生物标志物,ncRNA具有潜力.

研究的目的:

系统地评估CLL的ncRNA的诊断准确性.
评估miRNAs和CircRNAs作为CLL中的非侵入性生物标志物的性能.

主要方法:

在主要数据库中进行了全面的文献搜索,截至2024年1月.
使用 QUADAS-2 工具对包含的研究进行了偏差评估.
用GRADE方法评估诊断测试准确性的证据确定性.

主要成果:

对934名CLL患者的14项研究进行了分析,重点是miRNA和CircRNA.
组合的MiRNA显示出高的诊断性能 (灵敏度为0. 84,特异性为0. 98,AUC为0. 96).
CircRNAs表现中等 (灵敏度为0. 69,特异性为0. 77,AUC为0. 80),证据的确定性非常低或很低.

结论:

作为CLL的非侵入性诊断生物标志物,MiRNAs和CircRNAs具有前景.
与CircRNA相比,MiRNA具有更高的诊断潜力.
目前证据的确定性很低,需要在未来对更大群体和标准化方案进行严格的研究.

关键词:

美国慢性淋巴细胞白血病在CircRNA中诊断情况一个LncRNAs 非编码RNA 微型RNAs 其他

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相关概念视频

lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...