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净化和活性测定N端乙转移酶D

Yi-Hsun Ho1, Emma K Seipp1, Rong Huang1

  • 1Borch Department of Medicinal Chemistry and Molecular Pharmacology, Purdue Institute for Drug Discovery, Purdue University Center for Cancer Research, Purdue University, West Lafayette, IN, United States.

Methods in enzymology
|August 31, 2025
PubMed
概括

我们开发了两种测试方法来测量α-N-终端乙转移酶D (NatD) 的活性. 这些方法对于研究基因调节和发现新的NatD介导乙化抑制剂至关重要.

关键词:
马尔迪-MSNα-乙化乙化乙转移酶光检测

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Phase II Reactions: Acetylation Reactions

Acetylation, a phase II biotransformation reaction, introduces an acetyl group to drugs or their metabolites. Acetyltransferase enzymes facilitate this reaction, which resembles α-amino acid conjugation due to the addition of a functional group to the drug molecule.
The substrates for acetylation are typically drugs or their metabolites with an amino, sulfonamide, or hydrazine functional group. Acetylation can occur at several points in the drug molecule, including primary, secondary, and...
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