在单细胞和巨细胞中调节氧化应激的生物活性化合物
Renu Kushwaha1, Phaniendra Alugoju1, Naga Venkata Anusha Anthikapalli1
1Department of Biophysics, Faculty of Science, Palacký University, Šlechtitelů 27, 779 00, Olomouc, Czech Republic.
Scientific reports
|August 31, 2025
概括
植物化合物如欧蛋白和醇具有抗炎和抗氧化功效. 它们通过调节活性氧物种 (ROS) 和减少炎症标志物来帮助控制炎症.
科学领域:
- 免疫学和分子生物学
- 自然产品化学
背景情况:
- 炎症是一种关键的免疫反应,涉及反应性氧物种 (ROS).
- 过多的ROS会损害细胞,而中等水平对信号传递至关重要.
- 植物生物活性化合物对炎症和氧化应激具有潜在的治疗益处.
研究的目的:
- 评估基酸,油脂蛋白,西红和醇的抗炎和抗氧化能力.
- 研究它们对人类单细胞模型 (U-937和THP-1) 的影响.
- 评估它们与关键炎症点的分子相互作用.
主要方法:
- 细胞培养和U-937和THP-1细胞的分化.
- 对细胞形态和完整性的共聚焦显微镜.
- 基因和蛋白质表达分析 (GAPDH,IL-4,MPO,LOX-5) 通过RT-qPCR和西班牙血栓.
- 分子对接以预测与炎症蛋白的结合性.
主要成果:
- 没有观察到细胞毒性作用;GAPDH被确认为稳定的参考基因.
- 生物活性化合物增加了U-937细胞中的抗炎性Interleukin- 4 (IL- 4) 和减少了Myeloperoxidase (MPO) 表达.
- 基酸和醇降低了MPO活性;欧和醇抑制了5- Lipoxygenase (LOX-5) 的表达和活性.
结论:
- 植物衍生化合物,特别是欧蛋白和醇,具有显著的抗炎和抗氧化特性.
- 这些化合物调节与ROS相关的信号通路,并降低炎症标志物.
- 这些发现支持这些生物活性剂在治疗炎症和氧化应激方面的治疗潜力.
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