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Helcystogramma triannulella (Lepidoptera: Gelechiidae) 的染色体尺度基因组组

Ying Wang¹, Jinjun Cao², Baoni Qin¹

¹Henan International Joint Laboratory of Taxonomy and Systematic Evolution of Insecta, Henan Institute of Science and Technology, Henan, 453003, China.

Scientific data

|August 31, 2025

概括

这项研究为Helcystogramma triannulella,一个重要的农业害虫提供了第一个染色体规模的基因组组. 这种基因组资源有助于了解害虫生物学和制定有效的控制策略.

科学领域:

基因组学
农业昆虫学
害虫管理

背景情况:

海尔西斯托格拉玛 (Helcystogramma triannulella) 是一种对卷状植物作物的破坏性害虫,造成了巨大的经济损失.
有限的基因组资源阻碍了对H. triannulella生物学的理解和有效的害虫控制策略.

研究的目的:

为了生成Helcystogramma triannulella的第一个染色体规模的基因组组.
为研究和害虫管理提供有价值的基因组资源.

主要方法:

使用PacBio的HiFi和Hi-C测序技术.
通过 Hi-C 辅助脚手架实现了染色体规模的组装.
标注了蛋白质编码基因和重复元素.

主要成果:

产生了387.28 Mb的基因组组具有很高的连接性 (连接N50: 2.28 Mb,支架N50: 12.75 Mb).
91.77%的组合被固定在30个染色体上.
该组件非常完整 (BUSCO: 96.2%),包含14211个注释的蛋白质编码基因,其中49.02%被确定为重复元素.

结论:

染色体尺度基因组组合H. triannulella是一个显著的进步.
这种基因组资源将有助于未来的害虫生物学研究和针对性害虫管理策略的开发.

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