Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Chronic Pancreatitis I: Introduction01:24

Chronic Pancreatitis I: Introduction

148
The pancreas, an elongated and flat gland situated behind the stomach, serves a vital function in digesting food and managing blood sugar levels.
Pancreatitis is the inflammation of the pancreas, which occurs when the immune system becomes active and causes swelling, pain, and disruptions in organ function. Pancreatitis can manifest as either an acute or chronic condition.
Acute pancreatitis arises suddenly and lasts for a brief duration, while chronic pancreatitis is a long-term affliction...
148

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Intergenerational transmission of anxiety: A comparison of transdiagnostic and disorder-specific pathways.

JCPP advances·2026
Same author

The mediating roles of the challenge appraisal in the relationship between the coach-athlete relationship and adolescent athletes' burnout.

Frontiers in psychology·2026
Same author

Co-mutations of CTNNB1 and PTEN drive aggressive tumor progression in endometrial cancer.

Disease models & mechanisms·2026
Same author

Real-World Outcomes of Upadacitinib vs Risankizumab Among Anti-Tumor Necrosis Factor-Exposed Patients With Crohn's Disease.

Clinical gastroenterology and hepatology : the official clinical practice journal of the American Gastroenterological Association·2026
Same author

AI-driven discovery of bronopol for the treatment of pulmonary fibrosis via inhibition of fibroblast activation.

Life sciences·2026
Same author

Disentangling Degradation Mechanisms of Noble Metal-Coated Titanium Porous Transport Layers for Water Electrolyzers.

ACS applied materials & interfaces·2026

相关实验视频

Updated: Sep 9, 2025

Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells
09:40

Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells

Published on: June 20, 2018

17.8K

靠近管状细胞的PCAF损失通过促进部分上皮细胞转化为介质细胞而加剧纤维化.

Hyunsik Kim1, Jae-Hwan Kwon1, Sun-Ho Lee1

  • 1Department of Biochemistry and Molecular Biology, Graduate School of Medical Science, Brain Korea 21 Project, Yonsei University College of Medicine, Seoul, Republic of Korea.

Experimental & molecular medicine
|August 31, 2025
PubMed
概括

在纤维化的脏中,p300/ CBP相关因子 (PCAF) 水平下降. 在细胞中恢复PCAF可防止纤维化进展,突出显示PCAF是慢性病的潜在治疗点.

更多相关视频

Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium
08:46

Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium

Published on: September 1, 2015

9.9K
Identification of the Source of Secreted Proteins in the Kidney by Brefeldin A Injection
10:15

Identification of the Source of Secreted Proteins in the Kidney by Brefeldin A Injection

Published on: November 10, 2021

1.9K

相关实验视频

Last Updated: Sep 9, 2025

Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells
09:40

Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells

Published on: June 20, 2018

17.8K
Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium
08:46

Implementing Patch Clamp and Live Fluorescence Microscopy to Monitor Functional Properties of Freshly Isolated PKD Epithelium

Published on: September 1, 2015

9.9K
Identification of the Source of Secreted Proteins in the Kidney by Brefeldin A Injection
10:15

Identification of the Source of Secreted Proteins in the Kidney by Brefeldin A Injection

Published on: November 10, 2021

1.9K

科学领域:

  • 肝脏病学
  • 分子生物学
  • 细胞生物学

背景情况:

  • 纤维化是慢性病的一个标志,影响全球人口的10-14%.
  • 纤维化的基本分子机制尚不完全理解,需要新的治疗策略.
  • 目前对CKD和相关纤维化的治疗方法有限.

研究的目的:

  • 研究p300/CBP相关因子 (PCAF) 在纤维化病变的作用.
  • 确定PCAF是否可以作为缓解纤维化的治疗点.

主要方法:

  • 在纤维化组织中评估PCAF水平.
  • 在近接管状细胞中使用PCAF特异性淘汰和过度表达模型.
  • 在小鼠中使用单侧尿管阻塞和叶酸诱导的纤维化模型.
  • 研究了PCAF对表皮细胞转化为介质细胞转化 (EMT) 和粘附结基因表达的影响.
  • 研究了TGF-β信号对PCAF稳定性的影响.

主要成果:

  • 在纤维化组织中观察到PCAF水平的降低.
  • 在近端管状细胞中发生的PCAF敲击导致纤维化.
  • 在体内,PCAF过度表达减弱纤维化.
  • PCAF通过激活附着结基因来抑制近接管细胞EMT.
  • 在纤维化条件下,TGF-β信号促进PCAF溶酶体降解,降低其水平.

结论:

  • 作为纤维化的关键负调节剂.
  • 通过促进EMT,PCAF缺乏会加速纤维化.
  • 针对PCAF可能为治疗慢性病和纤维化提供一种新的治疗方法.