相关实验视频

Updated: Sep 9, 2025

Zebrafish Model of Neuroblastoma Metastasis

Zebrafish Model of Neuroblastoma Metastasis

Published on: March 14, 2021

与CBL的SPRY1相互作用促进维门稳定,以增强威尔姆斯瘤的恶性进展和转移

Fan Huang¹, Hongjie Gao², Zhiyi Lu¹

¹Department of Pediatric Surgery, Qilu Hospital of Shandong University, Jinan, China.

Annals of surgical oncology

|August 31, 2025

概括

高SPRY1表达通过CBL相互作用稳定维门丁,激活EMT通路,促进威尔姆斯瘤 (WT) 的生长. 针对SPRY1-CBL-vimentin轴提供了一个潜在的WT治疗策略.

科学领域:

癌症学
分子生物学
癌症研究

背景情况:

在各种瘤中,SPRY1 (Sprouty家族) 过度表达,与预后不佳和转移相关.
目前尚不清楚SPRY1在威尔姆斯瘤 (WT) 发生中的作用.
这项研究旨在澄清SPRY1在威尔姆斯瘤发育中的作用.

研究的目的:

研究SPRY1在威尔姆斯瘤 (WT) 瘤发生中的功能作用.
在WT患者中确定SPRY1表达的临床意义.
阐明SPRY1促进WT进展的分子机制.

主要方法:

对SPRY1表达和显著性的公共数据集和临床WT样本的分析.
在体外和体内测试 (CCK8,穿孔,伤口愈合,异种移植模型) 来评估SPRY1的生物功能.
共同免疫沉 (Co-IP),芯片-qPCR和功能实验以确定SPRY1的下游目标和调控机制.

主要成果:

高SPRY1表达与WT患者和细胞系的预后不佳有关.
在体外和体内,SPRY1 抑制了 WT 细胞的增殖,迁移和侵入.
通过SPRY1与E3无素结合酶CBL相互作用,破坏CBL-维门丁结合并促进维门丁积累,从而激活EMT通路.

结论:

通过与CBL结合,SPRY1积累稳定了维门丁,导致EMT通路过活化和威尔姆斯瘤致癌.
SPRY1-CBL-vimentin轴代表了威尔姆斯瘤治疗的潜在治疗标.

关键词:

中央银行芽 1个维门一个EMT 在地化威尔姆斯瘤

更多相关视频

Characterization of Cell Membrane Extensions and Studying Their Roles in Cancer Cell Adhesion Dynamics

Characterization of Cell Membrane Extensions and Studying Their Roles in Cancer Cell Adhesion Dynamics

Published on: March 26, 2018

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis

Quantitative PCR-based Assay to Measure Sonic Hedgehog Signaling in Cellular Model of Ciliogenesis

Published on: January 31, 2025

相关概念视频

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...

Tumor Progression

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...

Canonical Wnt Signaling Pathway

Canonical Wnt Signaling Pathway

The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...

Destabilization of Microtubules

Destabilization of Microtubules

The destabilization of microtubules can occur during different stages of the microtubule lifecycle, such as nucleation or elongation. It can take place at either end of the microtubule or in the microtubule lattices as a whole. The lifespan of individual microtubules within a cell varies according to the cell type and stage of the cell cycle. During interphase, the lifespan of the microtubule is about 30 minutes, while during cell division, it is about 15 minutes. In axonal microtubules of...

Induced Pluripotent Stem Cells

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...

Microtubule Instability

Microtubule Instability

Microtubules are hollow cylindrical filaments having a diameter of approximately 25 nm and a length that varies from 200 nm to 25 μm. GTP-bound tubulin subunits form αβ-heterodimers for microtubule assembly. These core building blocks interact longitudinally, polymerizing into protofilaments. The protofilaments then interact with one another through lateral bonding forces to form stable cylindrical microtubules. These cylindrical filaments are dynamic as they undergo repeated...