在外围CD8T细胞中Themis的作用
Masayuki Kitajima1, Toshiyuki Okada1, Kenta Nakano2
1Department of Immunology and Pathology, Research Institute National Center for Global Health and Medicine, 1-7-1 Kounodai, Ichikawa-shi, Chiba, 272-8516 Japan.
International immunology
|September 1, 2025
概括
泰米斯蛋白调节外围CD8T细胞中的T细胞受体信号,影响过敏性皮肤炎症. 它的缺席会降低CD8T细胞的反应,而它的存在会增强反应.
科学领域:
- 免疫学
- T细胞生物学
- 过敏性炎症
背景情况:
- 甲状腺对于胸腺T细胞的发育至关重要.
- 在过敏性皮肤炎症期间,其对外围T细胞受体 (TCR) 反应的作用尚不清楚.
研究的目的:
- 研究Themis在外围T细胞中的功能,特别是CD8T细胞介导的过敏皮肤炎症.
- 确定Themis是否影响CD8和CD4T细胞中的TCR信号值.
主要方法:
- 产生的泰莫西芬诱导的泰米斯条件淘汰 (cKO) 鼠标.
- 使用触角挑战 (CD8的DNFB,CD4的FITC/DBP) 来诱导过敏性皮肤炎症.
- 评估了T细胞透,细胞因子产生 (IFNγ),转录因子表达 (T-bet,Eomes) 和体外TCR刺激的增殖.
主要成果:
- 在cKO小鼠中,themis删除显著降低了DNFB诱导的耳朵胀,CD8T细胞透,IFNγ产生和T-bet/ Eomes表达.
- 转基因Themis过度表达增强了DNFB诱导的反应.
- 泰米斯缺陷没有改变FITC/ DBP诱导的CD4T细胞介导炎症.
- 在体外,缺乏Themis的CD8T细胞的增殖和IFNγ的产生下降,而CD4T细胞没有受到影响.
- 在Themis缺少的CD8T细胞中,SHP-1/2抑制恢复了IFNγ的产生.
- 缺少Grb2结合点的突变Themis模仿了Themis的cKO表型.
结论:
- 在过敏性皮肤炎症期间,Themis在调节外围CD8T细胞反应方面发挥着至关重要的作用.
- 通过调节 CD8 T 细胞中的 TCR 信号的值,依赖于 Grb2 结合.
- 在这种情况下,Themis似乎没有显著影响CD4T细胞的反应.
相关概念视频
T Cell Types and Functions
1.4K
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
1.4K
Antigens Involved in Adaptive Immunity
667
An antigen is any substance the immune system identifies as foreign and potentially harmful to the body, prompting an immune response. Antigens have two functional properties: immunogenicity and reactivity. Immunogenicity is the ability of an antigen to stimulate a specific immune response. At the same time, reactivity describes the antigen's ability to react with the cells and antibodies produced in response to it.
Complete Antigens
Complete antigens possess both immunogenicity and...
Complete Antigens
Complete antigens possess both immunogenicity and...
667
T Cell Activation and Clonal Selection
4.5K
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
4.5K
Hypersensitivities
1.0K
Hypersensitivity, also known as a hypersensitivity reaction or allergic reaction, is a condition where the body's immune system reacts abnormally to a foreign substance. Such substances, that cause hypersensitivity are referred to as an allergen, could be something typically harmless to most people, like pollen or certain foods.
Types of Hypersensitivities
Hypersensitivity reactions are categorized into four types: Type 1, Type 2, Type 3, and Type 4. Each type has a distinct mechanism...
Types of Hypersensitivities
Hypersensitivity reactions are categorized into four types: Type 1, Type 2, Type 3, and Type 4. Each type has a distinct mechanism...
1.0K
Cytotoxic T Cells-mediated Immune Response
1.9K
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
1.9K
Antigen Processing Pathways
1.3K
MHC molecules are key players in the immune response, enabling T cells to recognize and respond to specific antigens. They are present on the surface of all nucleated cells in the body and are instrumental in presenting antigens to T cells and activating them. T cells recognize the MHC-antigen complex and initiate an immune response. MHC class I and MHC class II are two main types of MHC molecules, each associated with a distinct antigen processing pathway.
MHC Class I: Presenting Endogenous...
MHC Class I: Presenting Endogenous...
1.3K

