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在伊朗伊斯法罕 (Isfahan) 隔离和环境检测Balamuthia mandrillaris

Sara Lesani¹, Soroush Mirzania¹, Abbasali Eskandarian¹

¹Department of Parasitology and Mycology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.

Environmental microbiology reports

|September 1, 2025

概括

在伊朗的环境样本中检测到了致死性脑炎的 Balamuthia mandrillaris. 土壤的患病率最高, 凸显了联合检测方法和公共卫生意识的必要性.

科学领域:

环境微生物学
寄生虫学
分子诊断

背景情况:

巴拉穆提亚 (Balamuthia mandrillaris) 是一种在全球范围内分布在环境中的病原性虫.
致命的脑炎是Balamuthia mandrillaris感染的一个已知的后果.
在伊朗的Balamuthia mandrillaris分布和检测数据有限.

研究的目的:

调查伊朗伊斯法罕的Balamuthia mandrillaris在环境中的存在情况.
为了比较基于培养和分子检测方法的Balamuthia mandrillaris.
确定该地区的Balamuthia mandrillaris潜在的环境储备.

主要方法:

在伊朗伊斯法罕收集了96个环境样本 (土壤,尘埃,水).
使用培养方法和针对线粒体16SrDNA的PCR对样本进行并行检查.
通过PCR阳性样本测序来确认物种.

主要成果:

在使用组合方法的环境样本中检测到了Balamuthia mandrillaris的15.6%.
土壤样本呈现出最高的流行率:24.2% (分子) 和21.2% (培养).
培养和分子方法显示补充性,依赖于矩阵的模式与实质性一致 (κ=0.72).

结论:

在伊斯法罕的各种环境来源中存在Balamuthia mandrillaris,土壤被确定为潜在的水库.
基于分子和培养的联合检测方法对于准确的识别至关重要.
建议对Balamuthia mandrillaris进行公共卫生宣传,即使在没有报告的临床病例地区.

关键词:

巴拉穆提亚大文化检测新出现的病原体自由生活的虫分子诊断

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