使用含有 Oregano 精油的聚合物纳米颗粒用于食品和环境应用的鼠类诺罗病毒的无活化
Beatriz Pereira Savi1, Catielen Paula Pavi1, Bianca da Costa Bernardo Port2
1Laboratory of Applied Virology, Department of Microbiology, Immunology and Parasitology, Federal University of Santa Catarina, Florianópolis, Santa Catarina State, Brazil.
Food and environmental virology
|September 1, 2025
概括
纳米封装香精油 (OEO) 有效地使小鼠诺病毒1 (MNV-1) 失活. 这种纳米技术方法增强了OEO
科学领域:
- 食品安全和环境微生物.
- 纳米技术和材料科学
- 自然产品的化学.
背景情况:
- 诺罗病毒引起广泛的胃肠炎疫情,由于它们的环境稳定性,它们作为食品和水污染物具有重大风险.
- Oregano 精油 (OEO) 具有强大的抗病毒特性,但面临降解,氧化和水溶性差等局限性.
- 纳米封装提供了一个解决方案来提高OEO的稳定性,可控释放和应用潜力.
研究的目的:
- 评估含有Origanum vulgare精油 (OEO) 的基索桑纳米颗粒对小鼠诺病毒1 (MNV-1) 的杀菌效果.
- 评估纳米封装OEO作为食品和环境应用的消毒剂的潜力.
主要方法:
- 基托桑基聚合物纳米颗粒的制备,其中包括OEO.
- 通过确定OEO纳米颗粒处理后MNV-1病毒感染率的降低 (TCID50/ml) 来评估杀菌活性.
- 对OEO和空白纳米颗粒的细胞毒性评估.
主要成果:
- 在所有测试度下,纳米封装的OEO显示对MNV-1有效的病毒失活.
- 最高的无活化率 (99.72%) 是通过未稀释的OEO纳米颗粒配方 (含有OEO的40mg/ml) 实现的.
- 白色纳米颗粒没有杀菌活性,纯OEO在多种度下表现为细胞毒性.
结论:
- 基索基纳米封装成功增强了香精油的杀毒潜力.
- 纳米封装OEO为环境和食品安全应用提供了有前途的生物技术消毒剂.
- 这种方法克服了纯OEO的局限性,提供了更好的稳定性和有效性.
相关概念视频
01:27Chemical Agents for Microbial Control
199
Chemicals play important roles in controlling microbial growth by targeting microbial structures and functions as sanitizers, antiseptics, disinfectants, and sterilants.Alcohols are commonly used sanitizers, effectively disrupting lipid membranes, which compromises cell integrity. They are also used as antiseptics and disinfectants due to their rapid action and versatility.Phenols and their derivatives phenolics , known for denaturing proteins and disrupting cell membranes, are particularly...
199
01:28Biological Methods for Microbial Control
199
Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
199
01:28Antimicrobial Effectiveness
156
The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
156