通过产生抗真菌挥发性有机化合物,Bacillus thuringiensis G-5有效抑制Codonopsis pilosula收获后的疾病
Rongxiu Mo1, Lijun Feng1, Fanjin Kong1
1College of Life Science, Northwest Normal University, Lanzhou, 730070, People's Republic of China.
Antonie van Leeuwenhoek
|September 1, 2025
概括
来自Bacillus thuringiensis G-5的挥发性有机化合物有效地抑制了Codonopsis pilosula的真菌生长. 化合物3--2-具有强烈的抗真菌活性,保护植物免受收获后的疾病.
科学领域:
- 农业科学
- 微生物学
- 植物病理学
背景情况:
- 由于储存不当,Codonopsis pilosula容易受到真菌感染的影响,从而降低了它的药用价值.
- 植物内生植物衍生挥发性有机化合物 (VOC) 是一个有前途的策略,用于管理收获后的植物疾病.
研究的目的:
- 评估来自Bacillus thuringiensis G-5的VOCs对Fusarium oxysporum F-3和Penicillium oxalicum F-5的抗真菌效果,这是C. pilosula的两个主要收获后病原体.
- 确定主要的抗真菌挥发性化合物并阐明它们的作用机制.
主要方法:
- 使用来自Bacillus thuringiensis G-5的VOC进行了真菌生长抑制试验.
- 使用扫描电子显微镜和化物染色来评估细胞损伤.
- 头部气体色谱-离子运动谱测定了挥发性化合物.
- 确定了特定化合物的最小抑制度 (MIC).
- 在体内实验中评估了C. pilosula的疗效.
主要成果:
- 来自G-5的VOC显著抑制了F-3 (94. 01%) 和F-5 (95. 37%) 的细胞生长.
- 挥发性有机物破坏了真菌细胞膜的完整性并改变了形态.
- 3 - 二被确定为主要抗真菌成分,F-3的MIC为40μL/ L,F-5的MIC为20μL/ L.
- 与G-5 VOC相比,3- 二在控制C. pilosula感染方面表现出较高的体内疗效.
结论:
- 来自Bacillus thuringiensis G-5的VOC,特别是3-二,是对C. pilosula的收获后真菌病原体的一种强有力的生物控制剂.
- 通过破坏真菌细胞膜的完整性来发挥3--2-的作用.
- 这项研究为维护C. pilosula的治疗质量和了解基于微生物的疾病管理提供了一种新方法.
相关概念视频
01:27Chemical Agents for Microbial Control
199
Chemicals play important roles in controlling microbial growth by targeting microbial structures and functions as sanitizers, antiseptics, disinfectants, and sterilants.Alcohols are commonly used sanitizers, effectively disrupting lipid membranes, which compromises cell integrity. They are also used as antiseptics and disinfectants due to their rapid action and versatility.Phenols and their derivatives phenolics , known for denaturing proteins and disrupting cell membranes, are particularly...
199
01:28Biological Methods for Microbial Control
199
Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
199
02:50Transgenic Plants
7.4K
Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
7.4K