Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Real Time RT-PCR02:57

Real Time RT-PCR

59.2K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
59.2K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Gamma SARS-CoV-2 variant of concern infection repercussions on pregnancy outcomes: A translational cohort study.

International journal of gynaecology and obstetrics: the official organ of the International Federation of Gynaecology and Obstetrics·2026
Same author

Transmission dynamics of Oropouche virus in Latin America and the Caribbean.

Nature medicine·2026
Same author

Evolution and spillover dynamics of yellow fever at the forest-urban interface in Brazil.

Nature microbiology·2026
Same author

Genomic characterization of Sabiá virus in Brazil, 2019-2020: Implications for diagnostics, virus evolution, and receptor binding.

PLoS neglected tropical diseases·2026
Same author

HIV serologic reactivity varies with time of ART initiation in persons on long-term ART.

Journal of clinical microbiology·2025
Same author

Dengue, chikungunya and Zika virus surveillance in blood donors in Brazil, 2019-2021.

Vox sanguinis·2025

相关实验视频

Updated: Sep 9, 2025

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
12:20

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

11.9K

差异容量光谱用于实时监测RNA放大

Steffane Q Nascimento1, Rodrigo M Iost2, Thiago C Oliveira1

  • 1São Carlos Institute of Chemistry (IQSC), University of São Paulo (USP), São Carlos, SP 13560-970, Brazil.

The journal of physical chemistry. B
|September 1, 2025
PubMed
概括

一种新的电化学方法,分电容光谱 (DCS),为病毒诊断提供实时RNA检测. 这种具有成本效益的技术提供了快速,高灵敏度的结果,不需要复杂的仪器.

更多相关视频

Author Spotlight: Advancing Real-Time cAMP Detection in Cells Using cADDis Biosensor
06:03

Author Spotlight: Advancing Real-Time cAMP Detection in Cells Using cADDis Biosensor

Published on: March 22, 2024

1.1K
Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings
09:36

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

Published on: February 3, 2021

5.0K

相关实验视频

Last Updated: Sep 9, 2025

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons
12:20

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

11.9K
Author Spotlight: Advancing Real-Time cAMP Detection in Cells Using cADDis Biosensor
06:03

Author Spotlight: Advancing Real-Time cAMP Detection in Cells Using cADDis Biosensor

Published on: March 22, 2024

1.1K
Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings
09:36

Open-Source Miniature Fluorimeter to Monitor Real-Time Isothermal Nucleic Acid Amplification Reactions in Resource-Limited Settings

Published on: February 3, 2021

5.0K

科学领域:

  • 生物医学工程
  • 分析化学
  • 分子诊断

背景情况:

  • 目前的RNA放大方法如PCR和RT-qPCR是昂贵而复杂的.
  • 需要更简单,更实惠,更实时的RNA检测技术.
  • 病毒诊断在很大程度上依赖于准确和快速的RNA检测.

研究的目的:

  • 引入差电容光谱 (DCS) 作为一种新的实时电化学方法来检测RNA.
  • 为了证明DCS与循环介导的同热放大对RNA量化的有效性.
  • 建立DCS作为现有RNA检测技术的成本效益和敏感替代品.

主要方法:

  • 使用柔性碳纤维电极监测电极-电解质接口电容的变化.
  • 应用形电流以产生独特的手臂肩膀图 (ASD),表明RNA放大.
  • 采用循环介导的同热放大用于指数级的RNA目标生成.
  • 使用COVID-19患者的临床样本验证了该方法.

主要成果:

  • 在同热条件下,DCS成功生成了独特的臂肩图,反映了RNA放大.
  • 观察到与放大RNA存在相关的显著指数和多项式容量变化.
  • 在不需要复杂仪器的情况下实现快速,高灵敏的RNA检测.
  • 证明了该方法在患者样本中检测SARS-CoV-2RNA的有效性.

结论:

  • 不同电容光谱为实时RNA诊断提供了一个变革性,可负担和可扩展的平台.
  • DCS消除了对复杂设备的需求,降低了诊断成本,提高了可访问性.
  • 这种电化学方法对疾病监测和临床应用,特别是病毒检测具有重大前景.