可转移元素驱动Brassica rapa和B. oleracea的进化并扰乱基因表达
Po-Xing Zheng1,2, Chia-Ying Ko1,2,3, Jheng-Yan Ou1,2
1Biotechnology Center of Southern Taiwan, Academia Sinica, Tainan, 711010, Taiwan.
The Plant journal : for cell and molecular biology
|September 1, 2025
概括
可转移元素 (TE) 推动了Brassica物种的基因组多样性. 它们的多样化分布和调节影响了植物的发育和应激反应,为作物改善提供了潜力.
科学领域:
- 植物基因组学
- 分子进化
- 生物信息学
背景情况:
- 可转移元素 (TE) 是植物基因组多样性和基因调节的关键驱动因素.
- 由于它们不同的化历史,Brassica rapa和B. oleracea为研究TE动态提供了有价值的模型.
研究的目的:
- 开发一种精细的TE分类方法,并系统地分析Brassica基因组中的TE.
- 研究TEs在基因组分化中的作用及其与基因调节和植物特征的关联.
主要方法:
- 开发了一种精细的 TE 分类方法.
- 对12个B. rapa和B. oleracea基因组进行了系统分析.
- 进行合成和转录组分析以评估基因-TE关联和表达变异性.
主要成果:
- 鉴定了1878个TE家族,其中49.5%是物种共享的,并突出了LTR逆转移体的特定物种扩展.
- 在B. oleracea根中具有独特的调节特征的热敏Ty1-copy家族 (Copia0035).
- 观察到显著的种内TE插入变异性,在B. rapa中加入特定的插入和在B. oleracea中保留的插入与形态型相关.
- 发现TE参与发育,生殖和应激反应途径,近位基因表达变异性增加,特别是在花发育和开花时间.
结论:
- 测试特异性对Brassica物种的基因组进化和差异化有显著的贡献.
- 试管婴儿会影响基因表达的变异性,特别是在与发育和压力相关的途径上.
- 在作物改进中,TEs是育种和基因组工程的宝贵资源.
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