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相关概念视频

Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

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相关实验视频

Updated: Jul 8, 2026

An Orthotopic Model of Serous Ovarian Cancer in Immunocompetent Mice for in vivo Tumor Imaging and Monitoring of Tumor Immune Responses
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与lentivirus shRNA-HOTAIR结合的弱化Salmonella typhimurium L形式有效地抑制了小鼠上皮卵巢癌的瘤生长和转移

Yunjie Zhang1, Ziqing Tang1, Wei Zhao1

  • 1Department of Microbiology, Bengbu Medical University, Bengbu, 233030, Anhui, People's Republic of China; Anhui Key Laboratory of Infection and Immunity, Bengbu Medical University, Bengbu, 233030, Anhui, People's Republic of China.

European journal of pharmacology
|September 1, 2025
PubMed
概括
此摘要是机器生成的。

与shRNA-HOTAIR治疗相结合的减弱型沙门氏菌L形式有效抑制了小鼠的卵巢癌生长和转移. 这种新型的细菌介导方法对治疗上皮卵巢癌 (EOC) 是有前途的.

关键词:
细菌的L形式卵巢上皮癌霍泰尔发生转移其他沙门氏菌瘤原产地

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科学领域:

  • 癌症学
  • 微生物学
  • 分子生物学

背景情况:

  • 卵巢上皮癌 (EOC) 的死亡率很高.
  • 像HOTAIR这样的长非编码RNA与瘤进展和上皮介质转变 (EMT) 有关.

研究的目的:

  • 在小鼠EOC上研究减弱型Salmonella typhimurium (ST) L形式与lentivirusshRNA-HOTAIR结合的抗瘤作用.
  • 探索这种联合治疗的潜在抗瘤机制.

主要方法:

  • 诱导减弱的ST VNP20009变成细菌的L形式.
  • 在体外评估EOC细胞入侵.
  • 使用TUNEL试验进行瘤生长,转移和亡的体内评估.
  • 通过免疫组织化学和qRT-PCR分析与瘤发生相关的蛋白质和mRNA表达 (TGF-β1,ZEB1,Vimentin).

主要成果:

  • 减弱的STL形式在体外抑制了EOC细胞的侵入.
  • 组合治疗显著增加了小鼠卵巢瘤ID8细胞的亡.
  • 与对照药和单剂疗法相比,联合疗法明显抑制了瘤生长和肺转移.
  • 观察到促瘤因子 (TGF-β1,ZEB1,维门丁) 的表达减少.

结论:

  • 与lentivirus shHOTAIR结合的减弱STL形式在抑制小鼠卵巢癌的瘤生长和转移方面表现出更高的疗效.
  • 这种组合策略代表了EOC中细菌介导瘤治疗的有前途的新生物方法.