作为潜在的食品初始培养物的乳酸细菌的选择和表征
Dor Zipori1, Merlin Brychcy1, Agnes Weiss2
1Department of Food Microbiology and Hygiene, Institute of Food Science and Biotechnology, University of Hohenheim, Garbenstrasse 28, 70599, Stuttgart, Germany.
Food microbiology
|September 1, 2025
概括
研究人员发现了能够产生有益细菌β-葡萄糖的新型乳酸细菌 (LAB) 菌株. 这些菌株对于食品发酵是安全的,为增强食品提供了潜力,特别是在以水果为基础的应用中.
科学领域:
- 食品微生物学
- 生物技术
- 发酵科学
背景情况:
- 乳酸细菌 (LAB) 在发酵食品中至关重要,其中一些产生的外聚糖 (EPS) 提高了食品的质地和功能.
- 细菌β-葡萄糖是一种来自LAB的特定EPS,具有理想的质特性和健康益处,使其在食品应用中具有价值.
研究的目的:
- 识别和描述能够生产用于食品发酵的β-葡萄糖的LAB菌株,特别是对于以水果为基础的产品.
- 评估选择的生产β-葡萄糖的LAB菌株的安全性和基因组特征,以作为初始培养物.
主要方法:
- 在246个LAB分离物中进行表型和基因型选.
- 具有编码β-葡萄糖合成酶的GTF-2基因的菌株的鉴定.
- 精选品种的基因组,代谢和安全性评估.
主要成果:
- 选择了六种生产EPS的LAB菌株,包括Lacticaseibacillus paracasei LTH 2407 (其属中第一个具有GTF-2的菌株).
- 基因组分析显示了适合多种食物基因组的菌株特异性代谢特征.
- 安全性评估证实没有毒素,抗微生物耐药性或显著的生物氨基生成基因.
结论:
- 选择的LAB菌株,特别是Lacticaseibacillus paracasei LTH 2407,显示出在食品发酵中安全使用的潜力.
- 这些生产β-葡萄糖的菌株是增强发酵食品的有希望的候选品,包括以水果为基础的产品.
- 需要进一步研究发酵性能和工业相关性.
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