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双相Hog1激活和转运器基因重编程使食物的酵母能够极度耐糖

Hong Guo¹, Qi Wang¹, Wenxi Lv¹

¹College of Food Science and Engineering, Northwest University, Xi'An, 710069, Shaanxi, China; Laboratory of Nutritional and Healthy Food-Individuation Manufacturing Engineering, Xi'An, 710069, Shaanxi, China; Research Center of Food Safety Risk Assessment and Control, Xi'An, 710069, Shaanxi, China.

Food microbiology

|September 1, 2025

概括

与Saccharomyces cerevisiae相比,Zygosaccharomyces rouxii的高葡萄糖存活率更高,因为它具有明显的Hog1激酶激活和糖载体基因调节. 这揭示了酵母异的适应策略.

科学领域:

* 微生物学和分子生物学
* 酵母生理学和应激反应
* 系统生物学和基因组学

背景情况:

* 蛋白激酶Hog1对于酵母透调节和细胞反应至关重要.
尽管共享Hog1激酶,但Zygosaccharomyces rouxii和Saccharomyces cerevisiae表现出不同的耐糖性.
了解这些差异是改善微生物耐糖性的关键.

研究的目的:

* 系统地比较酵母表型,Hog1酸化动力学,以及在极高葡萄糖压力下转录的特征.
* 为了阐明Z. rouxii和S. cerevisiae之间的糖适应策略的进化差异.
* 确定设计增强微生物耐糖性的潜在目标.

主要方法:

在高血糖压力下,酵母生存和细胞体积恢复的表型分析.
* 测量Hog1酸化动力学和Hsp70相关性
*转录基因分析以分析差异表达的基因 (DEG),特别是跨膜传递体.

主要成果:

在高葡萄糖压力下,Z. rouxii表现出延长的生存和体积恢复,而S. cerevisiae则经历了不可逆转的崩.
* Hog1激酶在Z. rouxii中显示过时的激活,而在S. cerevisiae中显示持续的激活.
* S. cerevisiae增加了高亲和力传输体 (HXT3,HXT4),而Z. rouxii则诱导了低亲和力传输体 (FFZ1,FFZ2).

结论:

* 独特的Hog1激活动态和糖载体的不同调节有助于酵母的不同透适应策略.
*Z. rouxii在极端糖应激中使用低亲和力传递器,与S. cerevisiae的高亲和力方法形成对比.
* 这些发现为酵母的进化差异提供了洞察力,并为增强微生物耐糖性提供了目标.

关键词:

霍格1激酶现象型类植物抗糖应激性转录组分析红糖

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