细胞壁完整性通路调节南极真菌Geomyces wnf-18c中的红色素分泌
Jiawen Du1, Yunxia Zhao1, Lingzong Meng1
1School of Chemistry and Chemical Engineering, Linyi University, Linyi, Shandong, 276005, China.
Food microbiology
|September 1, 2025
概括
研究人员从极地真菌中发现了一种新鲜的紫红色素, 操纵细胞壁完整性 (CWI) 基因,特别是rho1和bck1,显著增强了色素分泌.
科学领域:
- 微生物学
- 生物技术
- 自然产品化学
背景情况:
- 天然颜料比合成颜料具有安全优势,但面临稀缺性,特别是微生物来源.
- 卡尔米尼酸是一种高价值的天然色素, 但其供应有限.
- 极地微生物是未被充分探索的新型天然产品来源.
研究的目的:
- 从极地真菌中分离和描述一种新的天然色素.
- 通过突变生成来增强色素的产生,并研究底层的分子机制.
- 探索这种颜料作为卡尔米尼酸的替代品的潜力.
主要方法:
- 从极地土壤中分离和识别出一种颜料生成的真菌.
- 大气和室温等离子体 (ARTP) 突变因子改善.
- 针对细胞壁完整性 (CWI) 途径基因 (rho1,bck1,CHS3) 的基因删除和过度表达研究.
- 与CWI相关的细胞壁组成和基因表达的分析.
主要成果:
- 从一个极地真菌中分离出一种安全,无毒的紫色红色素,类似于卡米尼酸.
- 在ARTP突变过程中产生了高颜色的菌株.
- 通过影响细胞壁组件和应激反应,删除rho1或bck1显著增加色素分泌.
- 过度表达CHS3抑制了色素分泌,证实了细胞壁完整性的作用.
结论:
- 极地微生物是新型天然颜料的宝贵来源.
- 细胞壁完整性 (CWI) 是这种极性真菌颜料分泌的关键调节途径.
- 针对CWI基因提供了一种增强微生物色素生成的有希望的策略.
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