PpNAC转录因子调节ENDOPOLYGALACTURONASE等位基因,控制桃花的质地和结石
Yingjie Zhao1, Shouzheng Lü1, Yanyan Sun1
1College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China.
Plant physiology
|September 1, 2025
概括
桃子的果实质感,特别是融化的肉和自由石的特征,由内聚聚氨酸酶 (endoPG) 基因PGF和PGM控制. 基因表达和DNA甲基化调节这些关键的水果质量特征.
科学领域:
- 植物遗传学
- 果实生物学
- 分子遗传学
背景情况:
- 桃子 (Prunus persica L. Batsch) 的果实质感变化,特别是软化和融化,显著影响了营销和储存.
- 在F-M位点内存在两个并列的内聚聚氨酶 (endoPG) 基因,即PGF和PGM,它们决定了融化/非融化和自由石/石的特征.
研究的目的:
- 调查桃肉质感和石头粘附的基因和分子机制.
- 确定PGF-PGM类型对自由石化肉 (FMF),粘结石化肉 (CMF) 和粘结石非化肉 (CNMF) 类型的贡献.
主要方法:
- 分子标记分析以确定PGF-PGM类型.
- 对PGF和PGM的基因表达分析 (空间时间).
- 研究了PpNAC转录因子在EndPG基因调节中的作用.
- 全基因组二硫酸盐测序以评估DNA甲基化模式.
主要成果:
- 提升的PGF表达与FMF表型相关,具有明显的时空模式.
- PpNAC74和PpNAC87激活了FMF桃中的PGF转录.
- 两个PGM等位基因,PGMSer和PGMPhe,表现出功能分歧,分别影响CMF和CNMF表型.
- 在CMF中协同激活PGMSer转录.
- DNA甲基化调节与软化相关的基因, 影响肉体融化和自由石的特征.
结论:
- 阐明了控制桃子肉质和石头粘附的遗传和分子调节网络.
- 鉴定了关键转录因子 (PpNAC) 和它们在调节EndPG基因表达中的作用.
- 证明了DNA甲基化对肉质特征的作用.
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