通过新分离的Bacillus sp优化蛋白酶的产生. 从红海使用脱脂大豆蛋糕
Olfat E Amin1, Ahmed M Aboul-Enein2, Ibrahim S Abd-Elsalam1
1Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Research Institute, National Research Centre, El-Behooth St., Dokki, Giza, Egypt.
Scientific reports
|September 1, 2025
概括
研究人员从红海中分离出一种新的海洋细菌, 这种菌株使用脱脂大豆蛋糕有效地产生性蛋白酶,显示出可持续的生物处理潜力.
科学领域:
- 微生物学
- 生物技术
- 海洋科学
背景情况:
- 蛋白酶是重要的工业酶.
- 海洋环境是具有独特酶能力的新型微生物的丰富来源.
- 可持续的生物处理需要从成本效益高的基质有效地生产酶.
研究的目的:
- 隔离和描述能够产生性蛋白酶的海洋细菌.
- 确定最佳的有机基质和酶生产条件.
- 评估一种新的海洋细菌菌株的工业应用潜力.
主要方法:
- 从海水样本中分离细菌.
- 分离物的形态和生化特征.
- 用于细菌鉴定的16S rRNA测序.
- 使用各种有机废物基质进行酶活性测定.
- 优化发酵条件 (基质度,注射剂大小,pH,温度,).
主要成果:
- 分离了三种生产性蛋白酶的细菌菌株.
- 鉴定为Bacillus amyloliquefaciens NRC-IB-11 (GenBank加入PP034178.1) 的最强大的菌株与B. amyloliquefaciens MPA 1034具有99%的相似性.
- 脱脂大豆蛋糕是产生酶的最有效基质.
- 在优化条件下,蛋白酶的最大活性为590U/ml.
- 该研究证明了使用单元介质的可持续生物处理.
结论:
- 一种来自海洋的新型Bacillus菌株,B. amyloliquefaciens NRC-IB-11,表现出高性蛋白酶的生产力.
- 脱脂大豆蛋糕是节省成本的酶生产的合适基质.
- 这项研究提供了利用海洋微生物资源制造性蛋白酶的可持续方法.
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