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紫外线变异增强了Schizochytrium sp中的DHA生物合成. 通过代谢重编程

Jing Wen¹, Lingling Huang¹, Danping Wang¹

¹Key Laboratory of Animal Physiology & Biochemistry, Nanjing Agricultural University, Nanjing, Jiangsu, China.

Biotechnology journal

|September 2, 2025

概括

紫外线突变产生了一种高产的海洋藻类突变体,UV1-3,用于生产多可沙酸 (DHA). 这种突变通过协调代谢调节增强了DHA生物合成,提供了可持续的欧米茄-3来源.

科学领域:

海洋生物技术
微生物遗传学
生物化学工程

背景情况:

这种病的病名是Schizochytrium sp 是一种关键的海洋微藻类,用于生产必需的欧米茄-3脂肪酸 (DHA).
目前DHA的工业产量有限,因此需要提高压力以节省成本.
了解DHA生物合成的代谢调节对于增强藻类的产生至关重要.

研究的目的:

开发一个高产的工业菌株 Schizochytrium sp. 通过紫外线突变发生增强的多可沙酸 (DHA) 生产.
阐明在选择的突变菌株中改善的DHA生物合成的分子机制.
提供对可扩展微生物DHA生产的代谢流调节的见解.

主要方法:

通过紫外线突变生成了Schizochytrium sp的突变.
进行了转录组和代谢组分析,以比较野生型和突变型菌株.
使用定量实时PCR (RT-qPCR) 来验证基因表达水平.

主要成果:

与野生类型相比,一种稳定的突变物UV1-3被分离出来,其DHA产量增加了40. 34% (5. 01g/ l).
在UV1- 3中,代谢分析显示脂肪酸合成酶 (FAS) 途径基因的下调和多基基因合成酶 (PKS) 途径基因的上调.
观察到氧化酸化和三碳酸 (TCA) 循环活性降低,将代谢流转向DHA积累.

结论:

这种UV1-3突变是一种有前途的高产工业菌株,用于生产多可沙酸 (DHA).
对FAS,PKS,氧化化和TCA循环途径的协调调节促进了Schizochytrium sp中的DHA生物合成.
这项研究提供了对新陈代谢调节的基本见解,促进了可扩展和可持续的微生物omega-3生产.

关键词:

DHA 的使用精神分裂症脂肪酸生物合成转录组学紫外线突变发生

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In-vitro Mutagenesis

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

Spontaneous and Induced Mutations

Spontaneous and Induced Mutations

Spontaneous mutations arise infrequently during DNA replication due to errors in the process. A key factor behind these errors is tautomeric shifts in nitrogenous bases, where bases transition from keto to enol forms or amino to imino forms. This shift can alter base-pairing rules, leading to mutations. Additionally, reactive oxygen species (ROS) arising from aerobic metabolism can damage DNA, resulting in depurination (loss of a purine base) or depyrimidination (loss of a pyrimidine base).