诊断急性损伤中的PANoptosis相关基因:生物信息学,机器学习和验证
Zhen Chen1, Xiaogang Wang1, Yifan Shao1
1Departments of Urology, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu Province, China.
Annals of medicine
|September 2, 2025
概括
细胞死亡过程PANoptosis在急性损伤 (AKI) 中起着关键作用. 这项研究确定了特定的PANoptosis基因作为诊断AKI的潜在生物标志物,并作为未来治疗的目标.
科学领域:
- 分子生物学
- 免疫学
- 遗传学
背景情况:
- 急性损伤 (AKI) 是一种严重的疾病,涉及功能迅速丧失和炎症.
- 一种编程细胞死亡形式的PANoptosis对AKI的发展和进展作出了重大贡献.
- 了解与PANoptosis相关的基因的作用对于AKI研究至关重要.
研究的目的:
- 研究与PANoptosis相关的基因在AKI中的诊断和治疗潜力.
- 确定与AKI病理生理学相关的关键PANoptosis基因.
- 探索PANoptosis基因,免疫细胞透和潜在的药物点之间的关系.
主要方法:
- 分析了来自脏活检的转录组数据,以确定与AKI中的PANoptosis相关的差异表达基因 (DEGs).
- 用权重基因关联网络分析 (WGCNA) 来识别枢纽基因.
- 单个样本基因组丰富分析 (ssGSEA) 计算了PANoptosis得分和免疫细胞透.
- 机器学习算法和ROC分析选择了诊断特征基因,并调查了药物基因相互作用.
主要成果:
- 与PANoptosis相关的3460个DEG被确定,在细胞因子信号传递和细胞因子灭亡途径中得到丰富.
- 在AKI患者中发现了8个枢纽PANOPTOSIS基因,PANOPTOSIS得分显著更高.
- 选择了CASP8,CASP4,SFN,FAS和CASP1作为关键诊断标记,其中CASP8的预测值很高 (AUC=0. 850).
- 这些基因与免疫细胞透相关,并显示了潜在的药物关联,在小鼠模型中得到验证.
结论:
- 与PANoptosis相关的基因作为AKI的有前途的诊断生物标志物.
- 这些基因代表了AKI治疗的潜在治疗点.
- 这些发现强调了PANoptosis在AKI相关的免疫失调中的作用.
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