细菌乙转移酶效应器AopP2通过乙化保存转录因子触发西瓜的效应器免疫力
Jingjing Huang1, Peimin He1, Chen Zhong1
1Guangxi Key Laboratory of Agro-environment and Agro-product Safety, College of Agriculture, Guangxi University, Nanning, China.
Plant, cell & environment
|September 2, 2025
概括
研究人员使用一种玉米病原体, 激发植物对西瓜的细菌免疫力. 通过修改关键的转录因子来激活植物防御,
科学领域:
- 植物病理学
- 分子植物微生物相互作用
- 提高作物质量
背景情况:
- 由Paracidovorax citrulli (Pc) 引起的细菌性果斑 (BFB) 在全球严重影响西瓜产量.
- 瓜子对BFB的遗传耐药性有限,需要采用替代性疾病管理策略.
- 不适应的病原体相互作用可以引起植物免疫反应,从而提供耐药性繁殖的潜力.
研究的目的:
- 调查使用非适应病原体Paracidovorax avenae (Pa) 的效应剂在西瓜中诱导BFB免疫力的可能性.
- 在西瓜中识别特定的Pa型III效应体 (T3E) 触发效应体触发免疫力 (ETI).
- 阐明AopP2介导免疫的分子机制及其在西瓜防御中的作用.
主要方法:
- 在西瓜中使用Pseudomonas fluorescens效应器对宿主分析器 (EtHAn) 系统进行过渡表达.
- 评估编程细胞死亡 (PCD) 诱导和效应器酶活性.
- 通过基因沉默和短暂表达来研究转录因子ClTFIIB2在基底和ETI介导的耐药性的作用.
主要成果:
- 在西瓜中,Pa乙转移酶AopP2诱导的PCD取决于其酶活性.
- AopP2抑制了活性氧物种 (ROS) 爆发和酸 (SA) 信号,同时通过乙化稳定了ClTFIIB2,从而激活了ETI.
- ClTFIIB2对于基础Pc耐药性和AopP2诱导的PCD至关重要;其操纵增强了西瓜对Pc和BFB的耐药性.
结论:
- AopP2通过乙化ClTFIIB2来抑制模式触发免疫力,但同时触发ETI,揭示了一个保存的免疫节点.
- 不适应的病原体因子可以成为识别和激活植物免疫反应的有价值工具.
- 这项研究为西瓜免疫提供了新的见解,并提供了设计BFB抗性的潜在策略.
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