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Updated: Sep 9, 2025

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
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从单细胞和纳米级样本进行定量蛋白质组分析的液体色谱和质谱方法

Yuefan Wang1, Jongmin Woo1, Zhenyu Sun1

  • 1Department of Pathology, Johns Hopkins University, Baltimore, Maryland 21231, United States.

Analytical chemistry
|September 2, 2025
PubMed
概括

这项研究优化了液体染色体质谱 (LC-MS) 的单细胞蛋白质组学,使敏感的蛋白质识别和量化在皮克克到纳米克的水平. 改进的方法成功分析了HeLa和PC3细胞,揭示了蛋白质组的变化.

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相关概念视频

Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
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