Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Protein Diffusion in the Membrane01:24

Protein Diffusion in the Membrane

4.6K
Proteins show rotational as well as lateral diffusion across the membrane. The lateral diffusion of proteins was confirmed through the cell fusion experiment where mouse and human cells were fused, resulting in hybrid cells. When the human and mouse cells fused, the specific membrane proteins on human and mouse cells were marked with the red and green-fluorescent markers, respectively. Initially, the red and green fluorescence was located on the respective hemisphere of the cell. As time...
4.6K
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

2.3K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.3K
Overview of Protein Sorting and Transport01:45

Overview of Protein Sorting and Transport

11.9K
Eukaryotic cells have different membrane-bound organelles with distinct protein requirements. The process by which proteins are targeted to a specific organelle is called protein sorting.
Protein sorting can be of two types: signal-based sorting and vesicle-based trafficking. In signal-based sorting, specific amino acid sequences called sorting signals target proteins to the proper location inside the cell either via gated transport or by protein translocation.  In gated transport, folded...
11.9K
ER Retrieval Pathway01:45

ER Retrieval Pathway

3.9K
In the secretory pathway, vesicles transport proteins from one cellular compartment to another in forward transport to deliver the protein to its correct location. Occasionally, misfolded proteins and incorrect proteins escape their original compartments, and a retrieval pathway is used to return the escaped proteins to their original compartment.
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
3.9K
Diffusion01:12

Diffusion

198.5K
Diffusion is the passive movement of substances down their concentration gradients—requiring no expenditure of cellular energy. Substances, such as molecules or ions, diffuse from an area of high concentration to an area of low concentration in the cytosol or across membranes. Eventually, the concentration will even out, with the substance moving randomly but causing no net change in concentration. Such a state is called dynamic equilibrium, which is essential for maintaining overall...
198.5K
Amyloid Fibrils03:03

Amyloid Fibrils

9.8K
Amyloid fibrils are aggregates of misfolded proteins.  Under most circumstances, misfolded proteins are either refolded by chaperone proteins or degraded by the proteasome. However, in the case of a mutation or a disease, these proteins can accumulate to form large clusters and often further assemble to form elongated fibers, called fibrils. 
Amyloid deposits were observed as early as 1639 in the liver and the spleen.   In 1854, Rudolph Virchow performed iodine staining,...
9.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Rotational memory function of SPC/E water.

The Journal of chemical physics·2026
Same author

Dynamics of low-temperature water are driven by electrostatics.

The Journal of chemical physics·2026
Same author

Protein Electron Transfer in Solution, Protein Powders, and Electrode Confinement.

ACS omega·2026
Same author

Photosynthetic Reaction Center: A Nonergodic, Dynamically Anisotropic, and Nonlinear Charge-Transport Engine.

The journal of physical chemistry letters·2025
Same author

Aqueous Ion Mobility over a Broad Concentration Range.

Physical review letters·2025
Same author

Correction to "Remarkable Insensitivity of Protein Diffusion to Protein Charge".

The journal of physical chemistry letters·2025
Same journal

The influence of chirality on the macroscopic behavior of multiferroic smectic phases.

The Journal of chemical physics·2026
Same journal

Polaron transformed canonically consistent quantum master equation.

The Journal of chemical physics·2026
Same journal

The x-ray absorption spectrum of the propargyl radical C3H3●.

The Journal of chemical physics·2026
Same journal

Transient hydroperoxyalkyl intermediates (•QOOH) in isopentane oxidation. I. Conformer- and isomer-resolved infrared spectra.

The Journal of chemical physics·2026
Same journal

Transient hydroperoxyalkyl intermediates (•QOOH) in isopentane oxidation. II. Isomer-resolved unimolecular dynamics.

The Journal of chemical physics·2026
Same journal

Quantum state-to-state dynamics studies of the C(3P) + OH(X2Π) → CO(a3Π) + H(2S) reaction based on a new HCO(12A″) potential energy surface.

The Journal of chemical physics·2026
查看所有相关文章

相关实验视频

Updated: Sep 9, 2025

Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy
12:15

Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy

Published on: April 9, 2019

8.8K

蛋白质扩散的记忆功能

Setare Mostajabi Sarhangi1, Dmitry V Matyushov2

  • 1Department of Physics, Arizona State University, P.O. Box 871504, Tempe, Arizona 85287-1504, USA.

The Journal of chemical physics
|September 2, 2025
PubMed
概括
此摘要是机器生成的。

这项研究引入了一种新的"力路径",用于在模拟中计算扩散常数,提供比标准位移或速度方法更准确的方法. 这种新方法对系统大小的依赖性较小,改善了蛋白质扩散分析.

更多相关视频

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
14:43

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

11.7K
Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
10:20

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

8.3K

相关实验视频

Last Updated: Sep 9, 2025

Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy
12:15

Image Processing Protocol for the Analysis of the Diffusion and Cluster Size of Membrane Receptors by Fluorescence Microscopy

Published on: April 9, 2019

8.8K
Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions
14:43

Combining Single-molecule Manipulation and Imaging for the Study of Protein-DNA Interactions

Published on: August 27, 2014

11.7K
Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules
10:20

Single-Molecule Tracking Microscopy - A Tool for Determining the Diffusive States of Cytosolic Molecules

Published on: September 5, 2019

8.3K

科学领域:

  • 计算物理
  • 生物物理
  • 物理化学

背景情况:

  • 标准扩散常数的计算依赖于平均平方位移或速度自相关函数.
  • 这些方法不考虑涉及的随机力量的物理性质.
  • 使用柯克伍德方程的力路线解决了扩散粒子的限制.

研究的目的:

  • 制定和验证分子动力学 (MD) 模拟中计算扩散常数的力路径.
  • 将力路线的精度和系统大小依赖性与传统方法进行比较.
  • 使用绿色光蛋白和塑素突变物的MD模拟来研究蛋白质扩散.

主要方法:

  • 六种绿色光蛋白和塑素的分子动力学 (MD) 模拟.
  • 计算记忆函数以确定记忆时间.
  • 使用力路径和与速度/位移路径进行比较,应用柯克伍德方程.
  • 对扩散常数计算系统大小影响的分析.

主要成果:

  • 与标准方法相比,使用记忆时间的力路提供了更准确的扩散常数计算.
  • 克克伍德方程,当通过力路线应用时,将蛋白质扩散常数高估大约为四倍.
  • 通过速度/位移路径计算的扩散常数表现出强大的系统大小依赖,标准校正显示了蛋白质扩散的重大缺陷.
  • 从力路径中得出的扩散常数显示出最小的系统大小依赖性,产生大大独立于系统大小的校正值.

结论:

  • 强力路径为计算扩散常数提供了更强大,更准确的方法,特别是对于蛋白质,通过考虑强力的物理性质.
  • 传统计算扩散常数和对有限大小效应进行校正的方法对于蛋白质扩散是不够的.
  • 力量路线对系统大小的依赖性降低使其成为复杂生物系统中精确的扩散分析的优越方法.