微纤维增强活体肌肉结构与高度对齐的细胞组织的连续混合生物打印
Zhennan Qiu1,2,3, Zijie Meng1,4,2,3, Ayiguli Kasimu1,2,3
1State Key Laboratory for Manufacturing Systems Engineering, Xi'an Jiaotong University, Xi'an, 710049, China.
Advanced materials (Deerfield Beach, Fla.)
|September 2, 2025
概括
这项研究引入了一种连续的混合生物打印方法, 技术工程师机械匹配,细胞结合的组织具有与原生组织相似的特性.
科学领域:
- 生物材料科学
- 组织工程
- 复原医学
背景情况:
- 工程功能骨肌结构需要复制本地细胞外基质 (ECM) 和细胞组织.
- 现有的方法在实现机械匹配和精确的细胞对齐方面面临挑战.
研究的目的:
- 开发一种新的生物打印策略来制造活体复合肌肉结构.
- 实现机械匹配,高度对齐的多孔肌肉结构与本地类似的属性.
主要方法:
- 连续混合生物打印 (CHB) 结合了电水力学 (EHD) 打印和基于挤压的生物打印.
- 使用聚合物微纤维,牺牲凝和带有细胞的纤维素水凝制造结构.
- 使用生物打印水凝作为导电表面,以稳定地打印高面积微纤维.
主要成果:
- 通过CHB可以制造高达1厘米的结构完整的微纤维.
- 牺牲水凝的去除导致有可调节的,与原生肌肉类似的机械特性.
- 微纤维结构引导细胞诱导的异构重塑,形成高度对齐的肌细胞束.
- 对齐的肌细胞结构分化为多核肌管,增强肌肉特异性基因和蛋白质表达.
结论:
- CHB策略成功设计了具有异型机械特性和细胞组织的复合结构.
- 这种方法为创建功能性骨肌肉组织提供了一个有前途的平台.
- 该方法允许对细胞对齐进行精确控制,使周围或层特定对齐的结构成为可能.
相关概念视频
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De novo myogenesis, or the formation of muscle fibers, begins during the early embryonic stages. The skeletal muscle is formed from somites– blocks of embryonic cell layers. The somites are further divided into dermatomes, myotomes, sclerotomes, and syndetomes. Among these, the myotomes give rise to muscle fibers.
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription factors...
Muscle progenitor cells (MPCs) are formed from the myotomes. MPCs express genes that encode the transcription factors Pax3 and Pax7. Along with Pax 3/7, other transcription factors...
01:22Muscle Stimulation Frequency
The contraction strength of muscles is regulated by motor neurons, which modulate the frequency of action potentials dispatched to the motor units based on the body's requirements. This process of varying the muscle stimulation frequency allows muscles to contract with a force that is precisely tailored to the needs of the moment, whether lifting a feather or a heavy box.
Wave summation
At low firing rates, motor neurons induce individual twitch contractions in muscle fibers. These twitches...
Wave summation
At low firing rates, motor neurons induce individual twitch contractions in muscle fibers. These twitches...