艾弗麦克,艾玛麦克酸,可以杀死阳性细菌,并向细菌的细胞外
Rabih Ahmad1, Drew Lewis1, Lou Ann Verellen1
1London Research and Development Centre, Agriculture and Agri-Food Canada, Ontario, Canada.
Microbiology spectrum
|September 2, 2025
概括
之前认为对细菌无活性的阿维尔麦克丁具有抗菌性,特别是对格拉姆阳性细菌具有抗菌性. 这种药物向细胞包膜合成, 提供了针对细菌感染的药物的潜在用途.
科学领域:
- 微生物学与传染病
- 药物发现和开发
背景情况:
- 艾维美克丁和密尔贝米辛主要被称为杀虫剂,通常被认为缺乏抗菌活性.
- 目前的研究还没有广泛研究各种抗菌素化合物的抗菌潜力.
研究的目的:
- 选一组阿弗梅克丁和相关的密尔贝米辛,以检测它们对阳性和阴性细菌的抗菌活性.
- 阐明活性化合物的作用机制,重点关注乳甲酸对细菌细胞外的影响.
主要方法:
- 对六种对代表性细菌物种的抗菌感应性测试.
- 使用* Bacillus subtilis*进行细菌杀菌测定,宏分子合成抑制研究和转录组测序.
- 用细胞壁向抗生素进行突变选择,基因操纵 (CRISPRi) 和药物协同作用测试.
主要成果:
- 艾玛酸对几种格拉姆阳性细菌和超透性大肠杆菌表现出显著的活性.
- 发现埃玛酸对* Bacillus subtilis*具有杀菌作用,导致细胞溶解并抑制糖合成.
- 转录组分析显示细胞外应激反应的激活和甲基因生物合成基因的诱导.
结论:
- 艾维美克,特别是艾玛美克酸,具有显著的抗阳性活性,挑战了以前的假设.
- 艾玛酸的机制涉及向细菌细胞外,特别是糖合成.
- 这些研究结果表明,阿弗梅克丁可用于治疗格拉姆阳性细菌感染,并需要对其更广泛的生态影响进行研究.
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