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开发用于输送mRNA脂质纳米颗粒的微针贴片

Sophia H Sakers¹, B Pradeep K Reddy², Gianna Fiduccia²

¹Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University School of Medicine, Atlanta, GA, USA.

Drug delivery and translational research

|September 2, 2025

概括

微针贴片 (MNPs) 提供一种稳定,无痛的方式来输送含有mRNA的脂质纳米粒子 (LNPs). 优化配方和低温干燥是通过MNP有效传递mRNA的关键.

科学领域:

生物技术
纳米技术
接种疫苗

背景情况:

通过微针贴片 (MNPs) 传递mRNA有望实现无痛的注射,减少医疗保健需求,并提高热稳定性.
脂质纳米粒子 (LNP) 对于保护和传递mRNA至关重要.

研究的目的:

在MNP中研究mRNA-LNP的配方和制造策略.
优化MNP的制造和储存,以有效地传递mRNA和蛋白质表达.

主要方法:

系统评估mRNA-LNP度,配方pH值,辅料 (如聚乙烯醇) 和干燥温度.
在体外和体内评估mRNA-LNP大小,封装效率和光酶蛋白表达.
在MNP制造过程中测试各种聚合物和糖的mRNA-LNP稳定性.

主要成果:

在制造过程中初始mRNA-LNP度较高导致蛋白质表达优异.
在MNP制造过程中的酸性pH在体外表达得到改善,但在体内没有.
聚乙烯醇 (PVA) 有效稳定了mRNA- LNP,低温干燥 (5°C) 保存了mRNA功能.
在高温下,mRNA表达初始损失,但在加快条件下长期稳定.

结论:

确定了成功将mRNA- LNP纳入MNP的关键配方和制造参数.
优化的MNP显示出稳定mRNA和改善蛋白质表达的潜力,扩大了mRNA疗法的可用性.
这项研究为开发微针贴片输送系统的热稳定mRNA-LNP提供了路线图.

关键词:

脂质纳米粒子微针贴片通过皮肤输送在 mRNA 中

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