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用于精确和敏感的微RNA成像的近红外响应光增强DNA纳米设备

Yan Zhong^1,2, Zheng Li¹, Bo Li¹

¹Department of Chemistry and Chemical Engineering, Inner Mongolia University, Hohhot, 010020, China.

Materials today. Bio

|September 2, 2025

概括

我们开发了一种光增强型DNA纳米装置, 这种光激活系统提供精确的时空成像, 显著提高早期癌症检测能力.

科学领域:

生物医学工程
分子成像
纳米技术

背景情况:

微RNA (miRNA) 是癌症早期检测的关键生物标志物.
传统的DNA探针由于miRNA丰度低和非特异性激活而面临限制.
开发敏感和特定的miRNA检测方法对于临床应用至关重要.

研究的目的:

创建一个光增强DNA纳米装置 (PAD) 用于敏感和时空miRNA成像.
克服传统DNA探针在miRNA检测方面的局限性.
为了实现光门,在现场激活增强生物和医学成像.

主要方法:

通过将可光切割的DNA探针连接到上转化纳米粒子 (UCs) 来构建一个DNA纳米设备.
使用近红外 (NIR) 光远程激活发射紫外线的探针,触发miRNA检测.
实施了双循环放大策略,涉及目标miRNA,mRNA和DNA针用于信号增强.

主要成果:

与单循环放大相比,miRNA检测极限得到了20倍的改善.
已证明有效防止不必要的激活和最小化非特异信号.
在携带瘤的小鼠模型中通过NIR光激活成功执行了空间时间控制的miRNA成像.

结论:

该PAD系统为敏感和精确的miRNA检测提供了一种新方法.
光门的时空控制提高了DNA纳米设备在核酸成像中的适用性.
这项技术有助于提升早期癌症检测和生物成像.

关键词:

在现场激活可光切割的DNA探针信号放大上转化纳米粒子微RNA成像

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