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在Corynebacterium glutamicum中改造的无膜有机体,用于增强体生物合成和抗菌的产生

Manman Sun^1,2, Yimeng Zhao³, Rodrigo Ledesma-Amaro⁴

¹Key Laboratory of High Magnetic Field and Ion Beam Physical Biology, Hefei Institutes of Physical Science, Chinese Academy of Sciences, Hefei, 230031, China.

Synthetic and systems biotechnology

|September 2, 2025

概括

研究人员在Corynebacterium glutamicum中设计了液态蛋白质凝结物以提高代谢效率. 这种液相分离 (LLPS) 的新型应用增强了有价值化合物的产生,并提高了对抗菌的耐受性.

科学领域:

生物技术
合成生物学
微生物工程

背景情况:

通过液相分离 (LLPS) 形成的无膜有机体 (MLOs) 提高了微生物细胞工厂的代谢效率.
在工业细菌Corynebacterium glutamicum中使用LLPS驱动的MLOs仍然未被探索.

研究的目的:

在Corynebacterium glutamicum中设计LLPS驱动的MLOs.
评估这些MLOs对代谢途径和宿主细胞耐受性的影响.

主要方法:

在C. glutamicum中使用C. elegans LAF-1的RGG域形成液态蛋白质凝聚物.
压缩物形成条件的优化 (诱导前期,诱导剂度,培养温度).
对印地基因生物合成途径和抗菌 (AMP) 的表达应用MLOs.

主要成果:

在C. glutamicum中成功形成液态蛋白质凝聚物.
为高效的凝结物形成提供最佳条件.
通过LLPS介导的MLOs增强了印地基因的生产.
通过MLOs改善宿主细胞对有毒AMP (梅利丁,乳蛋白B) 的耐受性.

结论:

在Corynebacterium glutamicum中可以成功地设计LLPS介导的MLOs.
有机物增强特定的代谢途径并调节宿主细胞的毒性.
这项工作为使用C. glutamicum在工业生物技术中应用LLPS开辟了新的途径.

关键词:

抗菌葡萄糖菌印度素没有膜的隔间液体 - 液相分离

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