通过极化M1巨细胞和调节肠道微生物群,改善了基于益生菌的"盾牌"辅助益生菌
Yang Wang1,2, Xiaomin Su1,2, Yao Liu3
1Central Laboratory, First Affiliated Hospital, Institute (College) of Integrative Medicine, Dalian Medical University, Dalian 116011, China.
Acta pharmaceutica Sinica. B
|September 2, 2025
概括
新的共生水凝保护肠道中的益生菌. 这些囊增强了结肠癌治疗的抗瘤作用,并提高了化疗的疗效.
科学领域:
- 生物技术
- 癌症研究
- 微生物学
背景情况:
- 益生菌对于通过短链脂肪酸 (SCFA) 生产来治疗结肠癌至关重要.
- 在胃肠道 (GIT) 中提供和殖民益生菌是由于恶劣条件而具有挑战性的.
- 大肠癌患者经常建议服用益生菌补充剂以改善益生菌代谢.
研究的目的:
- 开发一种新型的共生水凝系统,用于增强益生菌的输送和结肠癌的抗瘤功效.
- 调查水凝屏蔽对GIT条件的保护机制.
- 阐明共生系统的协同抗瘤机制及其化学疗法的强化.
主要方法:
- 使用凝-胰岛素 (GI) 屏蔽来封装Lactobacillus reuteri (Lr) 的共生水凝囊.
- 评估Lr@GI对胃酸的耐药性及其在GIT中的生物活性/附着性.
- 对抗瘤效应的评估,包括反应性氧物种 (ROS) 生成,NLRP3炎症酶激活,M1巨分化,肠壁恢复和SCFA产生.
- 研究Lr@GI与5-FU的协同作用.
主要成果:
- 凝-胰岛素 (GI) 护罩成功地保护了GIT中的Lactobacillus reuteri (Lr).
- 通过诱导ROS,激活NLRP3,促进M1巨分化和恢复肠道屏障功能,Lr@GI显示出显著的抗瘤作用.
- 协生系统增强了SCFA的产生,并强化了5-Fluorouracil的抗瘤功效.
- 调节了肠道菌群,促进了SCFA的产生,有助于整体抗瘤作用.
结论:
- 合成生物囊 (Lr@GI) 提供了一种有前途的策略,用于保护益生菌并增强它们在结肠癌中的抗瘤活性.
- 胃肠道屏蔽有效地改善了肠道内益生菌的生存和功能.
- 这种系统为肠道微生物瘤治疗提供了一种协同方法, 改善化疗结果和肠道健康.
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