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Updated: May 12, 2026

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents
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在1. 3%的DMSO介导的HL-60细胞分化过程中,IMDM-20增强了中性特征

Jorge Andrés Cázares-Preciado1, José Antonio Cruz-Cárdenas1, Alejandra López-Arredondo1

  • 1Tecnológico de Monterrey, Escuela de Ingeniería y Ciencias, Av. Eugenio Garza Sada 2501 Sur, Tecnologico, 64849, Monterrey, Nuevo León, Mexico.

Biochemistry and biophysics reports
|September 2, 2025
PubMed
概括

这项研究比较了HL-60细胞分化协议,发现IMDM-20与DMSO是中性细胞样细胞研究的最佳选择. 它增强了对体外中性粒细胞生物学和实验可重复性的理解.

关键词:
文化媒体差异化HL-60 的情况中性细胞类细胞中性粒细胞

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Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...