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Updated: Sep 9, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
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双探针结合在现场混合与滚动圆放大高复合空间转录组

Sarah E Maguire1, Joel Credle2, Elizabeth M W Bertelson1

  • 1Portal Bioscience, LLC, Baltimore, MD, 21205, USA.

Biochemistry and biophysics reports
|September 2, 2025
PubMed
概括

这项研究引入了LISH-LnR,一个新的空间转录学方法. 它准确地绘制了已降解的甲固定和嵌组织中的mRNA,从临床样本中获得了更深入的生物学见解.

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In Situ Hybridization for the Precise Localization of Transcripts in Plants12:15

In Situ Hybridization for the Precise Localization of Transcripts in Plants

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相关概念视频

In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
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