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来自墨西哥的ToBRFV分离物的宿主相关变异和传播特征的基因组洞察

Erika Janet Zamora-Macorra¹, Daniel Leobardo Ochoa-Martínez², Claudia Yaritza Chavarín-Camacho¹

¹Invernadero de virus fitopatógenos. Universidad Autónoma Chapingo, Preparatoria Agrícola, Texcoco, Mexico.

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|September 2, 2025

概括

番茄棕果病毒 (ToBRFV) 是一个全球性威胁. 这项研究揭示了基因组稳定性与甲基转移酶域的变异,影响不同的宿主,并建议改进疾病管理策略.

科学领域:

植物病毒学
基因组学
分子生物学

背景情况:

番茄棕果病毒 (ToBRFV) 由于具有很高的传染性,对全球番茄和胡产量产生了重大影响.
了解ToBRFV的基因组特征和传播对于有效的疾病管理和生物安全至关重要.

研究的目的:

来自墨西哥的ToBRFV分离物的基因组和生物特性.
用100个全球ToBRFV基因组进行比较基因组分析,重点关注单核酸变体 (SNV).
研究基因关系和实验生物测试,包括种子传播,以了解ToBRFV的演化和宿主相互作用.

主要方法:

对100个全球ToBRFV基因组进行比较基因组分析.
基因分析以确定遗传接近和传播途径.
实验生物测试包括宿主敏感性测试和种子传播测试.

主要成果:

遗传学分析显示,墨西哥分离物与来自北美,以色列和中国的菌株密切相关,这表明它们有共同的传播途径.
基因组比较显示了整体序列稳定性,但在126kDa复制酶甲基转移酶域中发现了SNV.
在SNV中表现出宿主特定的模式,在番茄中保留了形状,在*Capsicum annum*,*Solanum nigrum*和*Citrullus lanatus*中表现出明显的变化.
生物测试证实了其他属宿主的敏感性,并证明了Nicotiana rustica*的低频种子传播.

结论:

ToBRFV表现出显著的宿主特异性变异的基因组保护,特别是在甲基转移酶域内.
甲基转移酶域可能会在各种宿主物种中受到不同的进化压力.
这些发现为加强对ToBRFV的风险评估,种子健康检测方案和全球生物安全措施提供了关键的见解.

关键词:

没有SNV 为了BRFV 农业生物安全基因组变异性主体特异性适应通过种子传播单核酸变体西红

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