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在体内感染期间,宿主和病原体的基因表达异质性增加

Anna A Pérez-Umphrey¹, Jeremy Miller², Edan R Tulman²

¹Department of Biological Sciences, Virginia Tech, Blacksburg, VA, USA.

bioRxiv : the preprint server for biology

|September 2, 2025

概括

之前的病原体暴露会在宿主和感染细菌中产生基因表达差异. 这种变异性影响宿主免疫反应和感染期间的病原体行为,影响疾病的动态.

科学领域:

免疫学
微生物学
基因组学

背景情况:

由于先前接触或接种疫苗而获得的免疫力会影响人口层面的疾病特征.
获得免疫对宿主生理环境和再感染病原体的影响尚不清楚.
研究宿主和病原体的基因表达异质性对于了解传染病动态至关重要.

研究的目的:

确定先前的病原体暴露是否会在感染期间诱导宿主和/或病原体的基因表达异质性.
研究这种异质性对宿主免疫反应和病原体行为的后果.
评估先前暴露剂量对基因表达变异性的作用.

主要方法:

在家用 (Haemorhous mexicanus) 中量化基因表达,先前暴露于Mycoplasma gallisepticum (MG) 的历史不同.
从宿主组织 (结膜,) 和感染病原体 (结膜) 收集的转录学数据.
对宿主基因表达数据进行功能丰富分析.

主要成果:

之前的MG暴露在宿主结膜中诱导了显著的基因表达异质性,但不是脏.
较低的先前暴露剂量导致重新挑战期间更大的组内基因表达异质性.
主体基因表达的变化与免疫反应有关,包括多样化的炎症反应.
感染病原体在之前暴露的宿主中也表现出更多的异质基因表达.

结论:

之前的宿主暴露可以在宿主和病原体中创造一个促进基因表达异质性的宿主环境.
基因表达的变化与遗传的变化一起,对于理解传染病的共同进化很重要.
这项研究强调了宿主-病原体相互作用的宿主内部变异性的重要性.

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