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一层一层的纳米粒子对外聚离子的影响蛋白质冠状形成

Tamara G Dacoba, Simone A Douglas-Green, Bhuvna Murthy

bioRxiv : the preprint server for biology

|September 2, 2025

概括

层对层 (LbL) 纳米颗粒的表面修饰影响蛋白质冠状形成,影响它们的细胞吸收和生物分布以改善药物输送.

科学领域:

生物材料科学
纳米技术
提供药物

背景情况:

纳米粒子 (NP) 的表面特性对于有针对性的传递至关重要.
在NP上形成的蛋白质冠状改变了它们的生物命运和治疗效果.
一层一层 (LbL) 的NP通过外层的多离子层提供可调整的表面特性.

研究的目的:

研究LbL NP外层对冠状蛋白组成和随后的生物相互作用的影响.
确定控制蛋白质冠状形成是否可以优化治疗应用的NP性能.

主要方法:

优化过以隔离LbLNP与蛋白质冠状.
质谱分析蛋白质冠状组成.
在体外细胞吸收研究 (巨细胞,卵巢癌细胞).
评估血清半衰期和生物分布的体内研究.

主要成果:

与PEG脂质体相比,阴性同聚 (PLD,PLE) 和PAA外层的蛋白质相关性较低.
在LbL NP类型中,冠状蛋白的组成有所不同,其中白蛋白,α-2-巨型球蛋白和阿波利波蛋白B是丰富的.
预先形成的蛋白质冠状体对巨细胞和癌细胞的NP吸收有不同的影响.
在体内,LbL NP外层显著影响了血清半衰期和生物分布.

结论:

可以设计LbLNP来调节蛋白质冠状形成.
了解NP-生物流体相互作用是开发临床可转化NP平台的关键.
LbL NPs的表面化学决定了蛋白质冠状和随后的体外/体内行为.

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