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Colloidal precipitates01:09

Colloidal precipitates

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The high insolubility of some precipitates can result in an unfavorable relative supersaturation. This can lead to colloidal particles with a large surface-to-mass ratio, where adsorption is promoted. For instance, in the precipitation of silver chloride, silver ions are adsorbed on the surface of the colloidal particles, forming a primary layer. This layer attracts ions of opposite charge (such as nitrate ions), forming a diffuse secondary layer of adsorbed ions. This electric double layer...
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Capillary Electrophoresis Mass Spectrometry Approaches for Characterization of the Protein and Metabolite Corona Acquired by Nanomaterials
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一层一层的纳米粒子对外聚离子的影响 蛋白质冠状形成

Tamara G Dacoba, Simone A Douglas-Green, Bhuvna Murthy

    bioRxiv : the preprint server for biology
    |September 2, 2025
    PubMed
    概括

    层对层 (LbL) 纳米颗粒的表面修饰影响蛋白质冠状形成,影响它们的细胞吸收和生物分布以改善药物输送.

    科学领域:

    • 生物材料科学
    • 纳米技术
    • 提供药物

    背景情况:

    • 纳米粒子 (NP) 的表面特性对于有针对性的传递至关重要.
    • 在NP上形成的蛋白质冠状改变了它们的生物命运和治疗效果.
    • 一层一层 (LbL) 的NP通过外层的多离子层提供可调整的表面特性.

    研究的目的:

    • 研究LbL NP外层对冠状蛋白组成和随后的生物相互作用的影响.
    • 确定控制蛋白质冠状形成是否可以优化治疗应用的NP性能.

    主要方法:

    • 优化过以隔离LbLNP与蛋白质冠状.
    • 质谱分析蛋白质冠状组成.
    • 在体外细胞吸收研究 (巨细胞,卵巢癌细胞).
    • 评估血清半衰期和生物分布的体内研究.

    主要成果:

    • 与PEG脂质体相比,阴性同聚 (PLD,PLE) 和PAA外层的蛋白质相关性较低.
    • 在LbL NP类型中,冠状蛋白的组成有所不同,其中白蛋白,α-2-巨型球蛋白和阿波利波蛋白B是丰富的.
    • 预先形成的蛋白质冠状体对巨细胞和癌细胞的NP吸收有不同的影响.
    • 在体内,LbL NP外层显著影响了血清半衰期和生物分布.

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    结论:

    • 可以设计LbLNP来调节蛋白质冠状形成.
    • 了解NP-生物流体相互作用是开发临床可转化NP平台的关键.
    • LbL NPs的表面化学决定了蛋白质冠状和随后的体外/体内行为.