十多年来在密歇根州的肠道细菌中追踪等离子体的传播
bioRxiv : the preprint server for biology
|September 2, 2025
概括
产生碳酶的肠道细菌 (KPC) 通过流行血统和AK975等广泛宿主等质体传播. 在不同物种中追踪等离子体对于了解医院中的抗菌素耐药性至关重要.
科学领域:
- 微生物学
- 基因组学
- 流行病学
背景情况:
- 产生碳酶的肠道细菌 (KPC) 是一个重大威胁,通过等离子体和流行病系传播.
- 了解KPC传播的动态对于抗生素耐药性 (AMR) 的防治至关重要.
研究的目的:
- 确定克隆传播与等离子体转移对密歇根州医疗机构十年来KPC负担的贡献.
- 在各种细菌物种中识别流行的KPC等离子体及其宿主.
主要方法:
- 来自密歇根州47个医疗机构的1,058个KPC生产分离物的全基因组测序 (2013-2022年).
- 对527个分离物进行长读测序,以实现精确的等离子体追踪.
- 使用MOB套件进行等离子体分析以确定等离子体类型和宿主范围.
主要成果:
- 确定了64种不同的KPC等离子体类型,其中AK975是最常见的 (27%的分离物).
- 在20个物种和92个序列类型中发现了AK975等离子体,其中70%是非流行性血统.
- 流行性血统占隔离物的46%,表现出不同等离子体载体和特定的KPC等离子体的显著克隆扩张.
结论:
- 通过具有特定等离子体的流行血统和AK975等等离子体在不同物种中的广泛传播,KPC传播.
- 跨物种的长度研究对医疗保健机构中AMR传播途径的全面了解至关重要.
- 传统的监测可能会忽略罕见物种或血统的KPC传播,强调需要全面的基因组监测.
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